Activation-induced polarized recycling targets T cell antigen receptors to the immunological synapse; involvement of SNARE complexes
| العنوان: | Activation-induced polarized recycling targets T cell antigen receptors to the immunological synapse; involvement of SNARE complexes |
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| المؤلفون: | Das, Vincent, Nal, Béatrice, Dujeancourt, Annick, Thoulouze, Maria-Isabel, Galli, Thierry, Roux, Pascal, Dautry-Varsat, Alice, Alcover, Andrès |
| المساهمون: | Biologie des Interactions Cellulaires, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Transduction du Signal et Plasticite Dans Le Systeme Nerveux, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Immunity Immunity, 2004, 20 (5), pp.577-88. ⟨10.1016/S1074-7613(04)00106-2⟩ Immunity, Elsevier, 2004, 20 (5), pp.577-88. ⟨10.1016/S1074-7613(04)00106-2⟩ |
| بيانات النشر: | HAL CCSD, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | MESH: Protein Transport, T-Lymphocytes, Receptors, Antigen, T-Cell, Vesicular Transport Proteins, Antigen-Presenting Cells, Fluorescent Antibody Technique, chemical and pharmacologic phenomena, Lymphocyte Activation, Image Processing, Computer-Assisted, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Lymphocyte Activation, MESH: Fluorescent Antibody Technique, MESH: Humans, MESH: Antigen-Presenting Cells, Cell Polarity, Membrane Proteins, hemic and immune systems, MESH: Receptors, Antigen, T-Cell, MESH: Vesi, MESH: Image Processing, Computer-Assisted, Endocytosis, Protein Transport, MESH: T-Lymphocytes, MESH: Endocytosis, MESH: Membrane Proteins, MESH: Cell Polarity, SNARE Proteins, MESH: SNARE Proteins |
| الوصف: | The mechanism by which T cell antigen receptors (TCR) accumulate at the immunological synapse has not been fully elucidated. Since TCRs are continuously internalized and recycled back to the cell surface, we investigated the role of polarized recycling in TCR targeting to the immunological synapse. We show here that the recycling endosomal compartment of T cells encountering activatory antigen-presenting cells (APCs) polarizes towards the T cell-APC contact site. Moreover, TCRs in transit through recycling endosomes are targeted to the immunological synapse. Inhibition of T cell polarity, constitutive TCR endocytosis, or recycling reduces TCR accumulation at the immunological synapse. Conversely, increasing the amount of TCRs in recycling endosomes before synapse formation enhanced their accumulation. Finally, we show that exocytic t-SNAREs from T cells cluster at the APC contact site and that tetanus toxin inhibits TCR accumulation at the immunological synapse, indicating that vesicle fusion mediated by SNARE complexes is involved in TCR targeting to the immunological synapse. |
| اللغة: | English |
| تدمد: | 1074-7613 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::73e14d10fff5bcbf2ffd649d3f07eb07Test https://hal-pasteur.archives-ouvertes.fr/pasteur-00137478Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....73e14d10fff5bcbf2ffd649d3f07eb07 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10747613 |
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