A metagenomic beta-glucuronidase uncovers a core adaptive function of the human intestinal microbiome
| العنوان: | A metagenomic beta-glucuronidase uncovers a core adaptive function of the human intestinal microbiome |
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| المؤلفون: | Hanane El oumami, Olivier Berteau, Marion Leclerc, Joël Doré, Karine Gloux, Fabienne Béguet |
| المساهمون: | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, French Ministry of Research |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2011, 108, pp.4539-4546. ⟨10.1073/pnas.1000066107⟩ Proceedings of the National Academy of Sciences of the United States of America (108), 4539-4546. (2011) |
| بيانات النشر: | HAL CCSD, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | intestinal microbiota, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Adaptation, Biological, functional metagenomics, Genome, glycosyl hydrolase, GUT MICROBIOME, RNA, Ribosomal, 16S, Cloning, Molecular, Intestinal Mucosa, Phylogeny, Glucuronidase, Genetics, 0303 health sciences, Multidisciplinary, biology, HYDROLYSIS, CROHNS-DISEASE, Intestines, ESCHERICHIA-COLI, BACTERIA, GEN. NOV, Ruminococcaceae, Firmicutes, Colloquium Papers, Genetic Vectors, Molecular Sequence Data, BIOLOGICAL-ACTIVITIES, Bacterial genome size, METABOLISM, RATS, 03 medical and health sciences, functional core, Humans, Microbiome, Amino Acid Sequence, FECAL MICROBIOTA, Gene, 030304 developmental biology, Gene Library, 030306 microbiology, Lachnospiraceae, Computational Biology, Sequence Analysis, DNA, biology.organism_classification, Metagenomics, Metagenome |
| الوصف: | In the human gastrointestinal tract, bacterial β- D -glucuronidases (BG; E.C. 3.2.1.31) are involved both in xenobiotic metabolism and in some of the beneficial effects of dietary compounds. Despite their biological significance, investigations are hampered by the fact that only a few BGs have so far been studied. A functional metagenomic approach was therefore performed on intestinal metagenomic libraries using chromogenic glucuronides as probes. Using this strategy, 19 positive metagenomic clones were identified but only one exhibited strong β- D -glucuronidase activity when subcloned into an expression vector. The cloned gene encoded a β- D -glucuronidase (called H11G11-BG) that had distant amino acid sequence homologies and an additional C terminus domain compared with known β- D -glucuronidases. Fifteen homologs were identified in public bacterial genome databases (38–57% identity with H11G11-BG) in the Firmicutes phylum. The genomes identified derived from strains from Ruminococcaceae, Lachnospiraceae, and Clostridiaceae. The genetic context diversity, with closely related symporters and gene duplication, argued for functional diversity and contribution to adaptive mechanisms. In contrast to the previously known β- D -glucuronidases, this previously undescribed type was present in the published microbiome of each healthy adult/child investigated ( n = 11) and was specific to the human gut ecosystem. In conclusion, our functional metagenomic approach revealed a class of BGs that may be part of a functional core specifically evolved to adapt to the human gut environment with major health implications. We propose consensus motifs for this unique Firmicutes β- D -glucuronidase subfamily and for the glycosyl hydrolase family 2. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0027-8424 1091-6490 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b90f4583ca2e5eca544820dce530460bTest https://hal.archives-ouvertes.fr/hal-01001013/documentTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b90f4583ca2e5eca544820dce530460b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00278424 10916490 |
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