Micro-distribution of uranium in bone after contamination: new insight into its mechanism of accumulation into bone tissue
| العنوان: | Micro-distribution of uranium in bone after contamination: new insight into its mechanism of accumulation into bone tissue |
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| المؤلفون: | Georges Boivin, Claude Vidaud, Brigitte Burt-Pichat, Damien Bourgeois, Xavier F. Le Goff, Luc Van Hoorebeke, Laszlo Vincze, Gerald Falkenberg, Jan Garrevoet, Daniel Meyer, Melissa A. Denecke, Pieter Tack |
| المساهمون: | Systèmes HYbrides pour la Séparation (LHyS), Institut de Chimie Séparative de Marcoule (ICSM - UMR 5257), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Ostéoporose et Qualité osseuse (Site Laennec), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-IFR62-Institut National de la Santé et de la Recherche Médicale (INSERM), Etude de la Matière en Mode Environnemental (L2ME), Universiteit Gent = Ghent University [Belgium] (UGENT), Department of Analytical Chemistry, Institut für Nukleare Entsorgung (INE), Karlsruher Institut für Technologie (KIT), Service de Biochimie et Toxicologie Nucléaire (SBTN), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Ostéoporose et Qualité Osseuse, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Universiteit Gent = Ghent University (UGENT) |
| المصدر: | Analytical and Bioanalytical Chemistry Analytical and Bioanalytical Chemistry, Springer Verlag, 2015, 407 (22), pp.6619-6625. ⟨10.1007/s00216-015-8835-7⟩ Analytical and Bioanalytical Chemistry, 2015, 407 (22), pp.6619-6625. ⟨10.1007/s00216-015-8835-7⟩ |
| بيانات النشر: | HAL CCSD, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, inorganic chemicals, chemistry.chemical_element, In Vitro Techniques, Bone tissue, complex mixtures, Biochemistry, Apatite, Analytical Chemistry, Rats, Sprague-Dawley, chemistry.chemical_compound, Bone cell, medicine, Animals, Tissue Distribution, Femur, ComputingMilieux_MISCELLANEOUS, Cartilage, Radiochemistry, technology, industry, and agriculture, Absorption, Radiation, Spectrometry, X-Ray Emission, [CHIM.MATE]Chemical Sciences/Material chemistry, Radiation Exposure, Uranium, Uranyl, Skeleton (computer programming), Rats, medicine.anatomical_structure, chemistry, visual_art, Biophysics, Bone Trabeculae, visual_art.visual_art_medium |
| الوصف: | After internal contamination, uranium rapidly distributes in the body; up to 20 % of the initial dose is retained in the skeleton, where it remains for years. Several studies suggest that uranium has a deleterious effect on the bone cell system, but little is known regarding the mechanisms leading to accumulation of uranium in bone tissue. We have performed synchrotron radiation-based micro-X-ray fluorescence (SR μ-XRF) studies to assess the initial distribution of uranium within cortical and trabecular bones in contaminated rats' femurs at the micrometer scale. This sensitive technique with high spatial resolution is the only method available that can be successfully applied, given the small amount of uranium in bone tissue. Uranium was found preferentially located in calcifying zones in exposed rats and rapidly accumulates in the endosteal and periosteal area of femoral metaphyses, in calcifying cartilage and in recently formed bone tissue along trabecular bone. Furthermore, specific localized areas with high accumulation of uranium were observed in regions identified as micro-vessels and on bone trabeculae. These observations are of high importance in the study of the accumulation of uranium in bone tissue, as the generally proposed passive chemical sorption on the surface of the inorganic part (apatite) of bone tissue cannot account for these results. Our study opens original perspectives in the field of exogenous metal bio-mineralization. |
| اللغة: | English |
| تدمد: | 1618-2642 1618-2650 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16a5f452b0a2072b782f3036314220ddTest https://hal.umontpellier.fr/hal-02000094Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....16a5f452b0a2072b782f3036314220dd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16182642 16182650 |
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