Physiological and metabolic consequences of autophagy deficiency for the management of nitrogen and protein resources in Arabidopsis leaves depending on nitrate availability
| العنوان: | Physiological and metabolic consequences of autophagy deficiency for the management of nitrogen and protein resources in Arabidopsis leaves depending on nitrate availability |
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| المؤلفون: | Anne Guiboileau, Jérémy Lothier, Marianne Azzopardi, Kohki Yoshimoto, Fabienne Soulay, Céline Masclaux-Daubresse, Anne Marmagne, Liliana Avila-Ospina |
| المساهمون: | Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA) |
| المصدر: | New Phytologist New Phytologist, Wiley, 2013, 199 (3), pp.683-694 New Phytologist, Wiley, 2013, 199 (3), pp.683-694. ⟨10.1111/nph.12307⟩ |
| بيانات النشر: | HAL CCSD, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | 0106 biological sciences, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Physiology, leaf senescence, Arabidopsis, Plant Science, 01 natural sciences, AMINO-ACIDS, Biomass, 2. Zero hunger, 0303 health sciences, aminopeptidase, biology, medicine.diagnostic_test, PROFILING APPROACH, nitrogen remobilization, REMOBILIZATION, carboxypeptidase, Biochemistry, 5-BISPHOSPHATE CARBOXYLASE OXYGENASE, Carbohydrate Metabolism, RNA Interference, RIBULOSE-1, EUGLENA-GRACILIS, nitrate availability, Nitrogen, Proteolysis, Blotting, Western, Protein degradation, 03 medical and health sciences, Ribosomal protein, Glutamate-Ammonia Ligase, GLUTAMINE-SYNTHETASE, Glutamine synthetase, medicine, Autophagy, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, PLANTS, 030304 developmental biology, selective autophagy, SENESCING LEAVES, Nitrates, Arabidopsis Proteins, Glutamate dehydrogenase, RuBisCO, DEGRADATION, biology.organism_classification, Carbon, Plant Leaves, Mutation, biology.protein, 010606 plant biology & botany, Peptide Hydrolases |
| الوصف: | Article de revue (Article scientifique dans une revue à comité de lecture); International audience; Autophagy is present at a basal level in all plant tissues and is induced during leaf ageing and in response to nitrogen (N) starvation. Nitrogen remobilization from the rosette to the seeds is impaired in autophagy mutants. This report focuses on the role of autophagy in leaf N management and proteolysis during plant ageing.Metabolites, enzyme activities and protein contents were monitored in several autophagy-defective (atg) Arabidopsis mutants grown under low and high nitrate conditions.Results showed that carbon (C) and N statuses were affected in atg mutants before any senescence symptoms appeared. atg mutants accumulated larger amounts of ammonium, amino acids and proteins than wild type, and were depleted in sugars. Over-accumulation of proteins in atg mutants was selective and occurred despite higher endopeptidase and carboxypeptidase activities. Specific over-accumulation of the ribosomal proteins S6 and L13 subunits, and of catalase and glutamate dehydrogenase proteins was observed. atg mutants also accumulated peptides putatively identified as degradation products of the Rubisco large subunit and glutamine synthetase 2 (GS2). Incomplete chloroplast protein degradation resulting from autophagy defects could explain the higher N concentrations measured in atg rosettes and defects in N remobilization.It is concluded that autophagy controls C:N status and protein content in leaves of Arabidopsis. |
| اللغة: | English |
| تدمد: | 0028-646X 1469-8137 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05d76d3c8ab4056b0979362c4d8290acTest https://hal.archives-ouvertes.fr/hal-01168786Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....05d76d3c8ab4056b0979362c4d8290ac |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 0028646X 14698137 |
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