Photodegradation of the Mycobacterium ulcerans Toxin, Mycolactones: Considerations for Handling and Storage
| العنوان: | Photodegradation of the Mycobacterium ulcerans Toxin, Mycolactones: Considerations for Handling and Storage |
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| المؤلفون: | Laurent Marsollier, Sarah Ghamrawi, Jérémie Babonneau, Estelle Marion, Camille Cano, Soizic Prado |
| المساهمون: | Centre de Recherche en Cancérologie Nantes-Angers (CRCNA), Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM)-Hôtel-Dieu de Nantes-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Laennec-Centre National de la Recherche Scientifique (CNRS)-Faculté de Médecine d'Angers-Centre hospitalier universitaire de Nantes (CHU Nantes), PRES Université Nantes Angers Le Mans (UNAM), Muséum national d'Histoire naturelle (MNHN), Univ Angers, Okina |
| المصدر: | PLoS ONE PLoS ONE, 2012, 7 (4), Non spécifié. ⟨10.1371/journal.pone.0033600⟩ PLoS ONE, Public Library of Science, 2012, 7 (4), Non spécifié. ⟨10.1371/journal.pone.0033600⟩ PLoS ONE, Vol 7, Iss 4, p e33600 (2012) |
| بيانات النشر: | HAL CCSD, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Buruli ulcer, Bacterial Diseases, Magnetic Resonance Spectroscopy, Light, [SDV]Life Sciences [q-bio], medicine.disease_cause, Biochemistry, Mass Spectrometry, chemistry.chemical_compound, Mycolactone, Buruli Ulcer, Chromatography, High Pressure Liquid, 0303 health sciences, Multidisciplinary, biology, Temperature, Bacterial Biochemistry, Bacterial Pathogens, [SDV] Life Sciences [q-bio], Infectious Diseases, Mycobacterium ulcerans, Medicine, Macrolides, Skin lesion, Research Article, Neglected Tropical Diseases, Bacilli, Ultraviolet Rays, Science, Virulence, Context (language use), Microbiology, Mycobacterium, 03 medical and health sciences, Diagnostic Medicine, medicine, Biology, 030304 developmental biology, Photolysis, 030306 microbiology, Toxin, Bacteriology, biology.organism_classification, medicine.disease, Emerging Infectious Diseases, chemistry |
| الوصف: | International audience; BackgroundMycolactones are toxins secreted by M. ulcerans, the etiological agent of Buruli ulcer. These toxins, which are the main virulence factors of the bacilli, are responsible for skin lesions. Considering their specificity for M. ulcerans and their presence in skin lesions even at early stages, mycolactones are promising candidates for the development of a diagnostic tool for M. ulcerans infection. Stability of purified mycolactones towards light and heat has not yet been investigated, despite the importance of such parameters in the selection of strategies for a diagnosis tool development. In this context, the effects of UV, light and temperature on mycolactone stability and biological activity were studied.Methodology/Principal FindingsTo investigate the effect of these physical parameters, mycolactones were exposed to different wavelengths in several solvents and temperatures. Structural changes and biological activity were monitored. Whilst high temperature had no effect on mycolactones, UV irradiation (UV-A, UV-B and UV-C) and sunlight exposure caused a considerable degradation, as revealed by LC-MS and NMR analysis, correlated with a loss of biological activity. Moreover, effect of UVs on mycolactone caused a photodegradation rather than a phototransformation due to the identification of degradation product.Conclusion/SignificanceThis study demonstrates the high sensitivity of mycolactones to UVs as such it defines instructions for storage and handling. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b2fb74894c3ea0fa436a726bf1b0b1bTest https://univ-angers.hal.science/hal-03388062/file/journal.pone_.0033600.pdfTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6b2fb74894c3ea0fa436a726bf1b0b1b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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