1H-NMR screening for the high-throughput determination of genotype and environmental effects on the content of asparagine in wheat grain
| العنوان: | 1H-NMR screening for the high-throughput determination of genotype and environmental effects on the content of asparagine in wheat grain |
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| المؤلفون: | Delia I. Corol, Jane L. Ward, Zoltán Bedo, Gilles Charmet, Catherine Ravel, Marianna Rakszegi, Michael H. Beale, Peter R. Shewry |
| المساهمون: | Department of Plant Biology and Crop Science, Rothamsted Research, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), Centre for Agricultural Research [Budapest] (ATK), Hungarian Academy of Sciences (MTA), European Commission in the Communities 6th Framework Programme, Biotechnology and Biological Sciences Research Council, Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Biotechnology and Biological Sciences Research Council (BBSRC)-Biotechnology and Biological Sciences Research Council (BBSRC) |
| المصدر: | Plant Biotechnology Journal Plant Biotechnology Journal, Wiley, 2016, 14 (1), pp.128-139. ⟨10.1111/pbi.12364⟩ |
| بيانات النشر: | HAL CCSD, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0106 biological sciences, Food industry, Edible Grain, Proton Magnetic Resonance Spectroscopy, Rain, Flour, Inheritance Patterns, Plant Science, heritability, 01 natural sciences, chemistry.chemical_compound, Soil, Surveys and Questionnaires, wheat, G × E, Asparagine, Cultivar, Food science, Amino Acids, Triticum, Research Articles, 2. Zero hunger, Temperature, 1H-NMR, food and beverages, 04 agricultural and veterinary sciences, 040401 food science, Acrylamide, Seeds, Carbohydrate Metabolism, Biotechnology, Research Article, Genotype, Biology, Environment, Gas Chromatography-Mass Spectrometry, 0404 agricultural biotechnology, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Dry matter, 1H‐NMR, G*E, business.industry, Plant Sciences, Phenotypic trait, Heritability, Biotechnology & Applied Microbiology, chemistry, metabolite profiling, business, Agronomy and Crop Science, 010606 plant biology & botany |
| الوصف: | Summary Free asparagine in cereals is known to be the precursor of acrylamide, a neurotoxic and carcinogenic product formed during cooking processes. Thus, the development of crops with lower asparagine is of considerable interest to growers and the food industry. In this study, we describe the development and application of a rapid 1H‐NMR‐based analysis of cereal flour, that is, suitable for quantifying asparagine levels, and hence acrylamide‐forming potential, across large numbers of samples. The screen was applied to flour samples from 150 bread wheats grown at a single site in 2005, providing the largest sample set to date. Additionally, screening of 26 selected cultivars grown for two further years in the same location and in three additional European locations in the third year (2007) provided six widely different environments to allow estimation of the environmental (E) and G x E effects on asparagine levels. Asparagine concentrations in the 150 genotypes ranged from 0.32 to 1.56 mg/g dry matter in wholemeal wheat flours. Asparagine levels were correlated with plant height and therefore, due to recent breeding activities to produce semi‐dwarf varieties, a negative relationship with the year of registration of the cultivar was also observed. The multisite study indicated that only 13% of the observed variation in asparagine levels was heritable, whilst the environmental contribution was 36% and the GxE component was 43%. Thus, compared to some other phenotypic traits, breeding for low asparagine wheats presents a difficult challenge. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1467-7644 1467-7652 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5dffa06f34a9dfb136343e2774d3285Test https://hal.archives-ouvertes.fr/hal-01276727Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a5dffa06f34a9dfb136343e2774d3285 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14677644 14677652 |
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