المؤلفون: Choi, Dong Wook, Roh, Yeon Jin, Kim, Seahyun, Lee, Hae Min, Kim, Minseo, Shin, Donghyuk, Park, Jong Ho, Cho, Yongmin, Park, Hee Ho, Ok, Yong Sik, Kang, Donghyun, Kim, Jin-Hong, Tarrago, Lionel, Danial, Nika, Gladyshev, Vadim, Min, Pil-Ki, Lee, Byung Cheon

المساهمون: Harvard Medical School Boston (HMS), Korea Polytechnic University (KPU), Yonsei University, Massachusetts General Hospital Boston, Kangwon National University, Seoul National University Seoul (SNU), Biodiversité et Biotechnologie Fongiques (BBF), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Chungnam National University (CNU), Korean government (2018M3A9F3055925 and 2018R1A1A1A05079386)

المصدر: ISSN: 0956-5663 ; Biosensors and Bioelectronics ; https://hal.inrae.fr/hal-03145199Test ; Biosensors and Bioelectronics, 2021, 178, pp.113031. ⟨10.1016/j.bios.2021.113031⟩.

مصطلحات موضوعية: Genetically-encoded fluorescent sensor, Free methionine-r-sulfoxide reductase, Methionine sulfoxide, Reactive oxygen species, Oxidative stress, Acute coronary syndrome, Reperfusion, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Aberrant production of reactive oxygen species (ROS) leads to tissue damage accumulation, which is associated with a myriad of human pathologies. Although several sensors have been developed for ROS quantification, their applications for ROS-related human physiologies and pathologies still remain problematic due to the unstable nature of ROS. Herein, we developed Trx1-cpYFP-fRMsr (TYfR), a genetically-encoded fluorescent biosensor with the remarkable specificity and sensitivity toward fMetRO (free Methionine-R-sulfoxide), allowing for dynamic quantification of physiological levels of fMetRO, a novel indicator of ROS and methionine redox status in vitro and in vivo. Moreover, using the sensor, we observed a significant fMetRO enrichment in serum from patients with acute coronary syndrome, one of the most severe cardiovascular diseases, which becomes more evident following percutaneous coronary intervention. Collectively, this study proposes that fMetRO is a novel biomarker of tissue damage accumulation in ROS-associated human pathologies, and that TYfR is a promising tool for quantifying fMetRO with potentials in versatile applications.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/33571808; hal-03145199; https://hal.inrae.fr/hal-03145199Test; PUBMED: 33571808; WOS: 000621207100006

الإتاحة: https://doi.org/10.1016/j.bios.2021.113031Test
https://hal.inrae.fr/hal-03145199Test

المؤلفون: Choi, Dong Wook, Roh, Yeon Jin, Kim, Seahyun, Lee, Hae Min, Kim, Minseo, Shin, Donghyuk, Park, Jong Ho, Cho, Yongmin, Park, Hee Ho, Ok, Yong Sik, Kang, Donghyun, Kim, Jin-Hong, Tarrago, Lionel, Danial, Nika, Gladyshev, Vadim, Min, Pil-Ki, Lee, Byung Cheon

المساهمون: Harvard Medical School Boston (HMS), Korea Polytechnic University (KPU), Yonsei University, Massachusetts General Hospital Boston, Kangwon National University, Seoul National University Seoul (SNU), Biodiversité et Biotechnologie Fongiques (BBF), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Chungnam National University (CNU), Korean government (2018M3A9F3055925 and 2018R1A1A1A05079386)

المصدر: ISSN: 0956-5663 ; Biosensors and Bioelectronics ; https://hal.inrae.fr/hal-03145199Test ; Biosensors and Bioelectronics, 2021, 178, pp.113031. ⟨10.1016/j.bios.2021.113031⟩.

مصطلحات موضوعية: Genetically-encoded fluorescent sensor, Free methionine-r-sulfoxide reductase, Methionine sulfoxide, Reactive oxygen species, Oxidative stress, Acute coronary syndrome, Reperfusion, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Aberrant production of reactive oxygen species (ROS) leads to tissue damage accumulation, which is associated with a myriad of human pathologies. Although several sensors have been developed for ROS quantification, their applications for ROS-related human physiologies and pathologies still remain problematic due to the unstable nature of ROS. Herein, we developed Trx1-cpYFP-fRMsr (TYfR), a genetically-encoded fluorescent biosensor with the remarkable specificity and sensitivity toward fMetRO (free Methionine-R-sulfoxide), allowing for dynamic quantification of physiological levels of fMetRO, a novel indicator of ROS and methionine redox status in vitro and in vivo. Moreover, using the sensor, we observed a significant fMetRO enrichment in serum from patients with acute coronary syndrome, one of the most severe cardiovascular diseases, which becomes more evident following percutaneous coronary intervention. Collectively, this study proposes that fMetRO is a novel biomarker of tissue damage accumulation in ROS-associated human pathologies, and that TYfR is a promising tool for quantifying fMetRO with potentials in versatile applications.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/33571808; hal-03145199; https://hal.inrae.fr/hal-03145199Test; PUBMED: 33571808; WOS: 000621207100006

الإتاحة: https://doi.org/10.1016/j.bios.2021.113031Test
https://hal.inrae.fr/hal-03145199Test

المؤلفون: Choi, Dong Wook, Roh, Yeon Jin, Kim, Seahyun, Lee, Hae Min, Kim, Minseo, Shin, Donghyuk, Park, Jong Ho, Cho, Yongmin, Park, Hee Ho, Ok, Yong Sik, Kang, Donghyun, Kim, Jin-Hong, Tarrago, Lionel, Danial, Nika, Gladyshev, Vadim, Min, Pil-Ki, Lee, Byung Cheon

المساهمون: Harvard Medical School Boston (HMS), Korea Polytechnic University (KPU), Yonsei University, Massachusetts General Hospital Boston, Kangwon National University, Seoul National University Seoul (SNU), Biodiversité et Biotechnologie Fongiques (BBF), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Chungnam National University (CNU), Korean government (2018M3A9F3055925 and 2018R1A1A1A05079386)

المصدر: ISSN: 0956-5663 ; Biosensors and Bioelectronics ; https://hal.inrae.fr/hal-03145199Test ; Biosensors and Bioelectronics, Elsevier, 2021, 178, pp.113031. ⟨10.1016/j.bios.2021.113031⟩.

مصطلحات موضوعية: Genetically-encoded fluorescent sensor, Free methionine-r-sulfoxide reductase, Methionine sulfoxide, Reactive oxygen species, Oxidative stress, Acute coronary syndrome, Reperfusion, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Aberrant production of reactive oxygen species (ROS) leads to tissue damage accumulation, which is associated with a myriad of human pathologies. Although several sensors have been developed for ROS quantification, their applications for ROS-related human physiologies and pathologies still remain problematic due to the unstable nature of ROS. Herein, we developed Trx1-cpYFP-fRMsr (TYfR), a genetically-encoded fluorescent biosensor with the remarkable specificity and sensitivity toward fMetRO (free Methionine-R-sulfoxide), allowing for dynamic quantification of physiological levels of fMetRO, a novel indicator of ROS and methionine redox status in vitro and in vivo. Moreover, using the sensor, we observed a significant fMetRO enrichment in serum from patients with acute coronary syndrome, one of the most severe cardiovascular diseases, which becomes more evident following percutaneous coronary intervention. Collectively, this study proposes that fMetRO is a novel biomarker of tissue damage accumulation in ROS-associated human pathologies, and that TYfR is a promising tool for quantifying fMetRO with potentials in versatile applications.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/33571808; hal-03145199; https://hal.inrae.fr/hal-03145199Test; PUBMED: 33571808; WOS: 000621207100006

الإتاحة: https://doi.org/10.1016/j.bios.2021.113031Test
https://hal.inrae.fr/hal-03145199Test