First molecular evidence of Babesia caballi and Theileria equi infections in horses in Cuba
| العنوان: | First molecular evidence of Babesia caballi and Theileria equi infections in horses in Cuba |
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| المؤلفون: | Alejandro Cabezas-Cruz, Sergio Luis Del Castillo Domínguez, Adrian Alberto Díaz-Sánchez, Carlos Yrurzun Estrada, Evelyn Lobo Rivero, Carlos Luiz Massard, Adivaldo Henrique da Fonseca, Ernesto Vega Cañizares, Belkis Corona-González, Marcus Sandes Pires |
| المساهمون: | Centro Nacional de Sanidad Agropecuaria (CENSA), Universidade Federal Rural do Rio de Janeiro (UFRRJ), Agrarian University of Habana, Partenaires INRAE, Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) |
| المصدر: | Parasitology Research Parasitology Research, Springer Verlag (Germany), 2018, pp.3109-3118. ⟨10.1007/s00436-018-6005-5⟩ |
| بيانات النشر: | HAL CCSD, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Tick infestation, Veterinary medicine, [SDV]Life Sciences [q-bio], ved/biology.organism_classification_rank.species, Polymerase Chain Reaction, 18S ribosomal RNA, Theileria equi, 0302 clinical medicine, Ticks, Theileria, Genotype, Babesia caballi, Prevalence, Phylogeny, Coinfection, Cuba, General Medicine, 3. Good health, 18S rRNA, Infectious Diseases, Female, 030231 tropical medicine, Babesia, Biology, Tick, 03 medical and health sciences, biology.animal, Babesiosis, parasitic diseases, medicine, Animals, Horses, nPCR, General Veterinary, ved/biology, Horse, Equidae, Equine piroplasmosis, medicine.disease, biology.organism_classification, bacterial infections and mycoses, Theileriasis, Tick Infestations, 030104 developmental biology, Insect Science, Parasitology, Cattle, Horse Diseases, Nested polymerase chain reaction |
| الوصف: | Contribution: Phylogenetic analysis and drafted the manuscript; International audience; Equine piroplasmosis is a disease of Equidae, including horses, donkeys, mules, and zebras, caused by either Theileria equi or Babesia caballi. This disease represents a serious problem for the horse industry and its control is critical for the international trade of horses. The objective of the present study was to detect B. caballi and T. equi infections in horses reared in western Cuba. Blood samples from 100 horses were tested for the presence of piroplasms by using Giemsa-stained blood smears and nested PCR (nPCR) assays targeting merozoite antigen genes of B. caballi (bc48) and T. equi (ema-1). All animals were inspected for the detection of tick infestation and tick specimens were collected for species identification. Erythrocyte inclusions were observed in 13 (13%) of the analyzed samples. nPCR analysis showed that 25 (25%) samples were positive for B. caballi, 73 (73%) for T. equi, and 20 (20%) showed dual infections. Only one tick species was found infesting horses, Dermacentor nitens. In addition, three nearly full-length sequences of T. equi 18S rRNA gene were obtained and subjected to phylogenetic analyses. This study reports a high prevalence of T. equi and B. caballi single and coinfections in horses in western Cuba. Molecular analysis of the 18S rRNA gene of T. equi suggested that different genotypes of this hemoparasite circulate in Cuba. To the best of our knowledge, this is the first report describing the molecular detection of B. caballi and T. equi in horses in Cuba. |
| اللغة: | English |
| تدمد: | 0932-0113 1432-1955 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d2ee5c9a6c4869bc75a0eab1dbc38c1Test https://hal.inrae.fr/hal-02623580Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....5d2ee5c9a6c4869bc75a0eab1dbc38c1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09320113 14321955 |
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