Description and genomic characterization of Massiliimalia massiliensis gen. nov., sp. nov., and Massiliimalia timonensis gen. nov., sp. nov., two new members of the family Ruminococcaceae isolated from the human gut
| العنوان: | Description and genomic characterization of Massiliimalia massiliensis gen. nov., sp. nov., and Massiliimalia timonensis gen. nov., sp. nov., two new members of the family Ruminococcaceae isolated from the human gut |
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| المؤلفون: | Pamela Afouda, Grégory Dubourg, Claudia Andrieu, Magali Richez, Didier Raoult, Enora Tomei, Pierre-Edouard Fournier, Niokhor Dione, Fabrizio Di Pinto, Jean-Christophe Lagier, Sory Ibrahima Traore |
| المساهمون: | Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, INSB-INSB-Centre National de la Recherche Scientifique (CNRS), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA), Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA) |
| المصدر: | Antonie van Leeuwenhoek Antonie van Leeuwenhoek, Springer Verlag, 2019, 112 (6), pp.905-918. ⟨10.1007/s10482-018-01223-x⟩ Antonie van Leeuwenhoek, 2019, 112 (6), pp.905-918. ⟨10.1007/s10482-018-01223-x⟩ |
| بيانات النشر: | HAL CCSD, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | DNA, Bacterial, Male, 0301 basic medicine, 030106 microbiology, Saudi Arabia, Biology, Microbiology, Feces, Young Adult, 03 medical and health sciences, Human gut, Genome Size, Genus, RNA, Ribosomal, 16S, Humans, Molecular Biology, Phylogeny, ComputingMilieux_MISCELLANEOUS, Base Composition, Clostridiales, Phylogenetic tree, Ruminococcus species, Genomics, General Medicine, Fecal bacteriotherapy, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Gastrointestinal Microbiome, 030104 developmental biology, Culturomics, Optimal growth, Family Ruminococcaceae, Genome, Bacterial |
| الوصف: | Using the culturomics approach, we isolated two strains, Marseille-P2963 and Marseille-P3753, from the intestinal microbiota of a 19-year-old healthy Saudi Arabian Bedouin male and from a 32-year-old healthy Senegalese male faecal transplant donor. Here, we studied their phenotypic, phylogenetic and genomic characteristics. Both strains were phylogenetically related, but different from Ruminococcus species. Bacterial cells were anaerobic, rod-shaped, non-spore-forming and not motile, with neither catalase nor oxidase activities. Their growth temperatures ranged from 28 to 45 °C, with an optimal growth at 37 °C. The genomes are 2,842,720 bp- and 2,707,061 bp-long respectively. The G + C contents are 47.18% and 46.90%, respectively. Based on these characteristics, we propose the creation of a new genus within the family Ruminococcaceae named Massiliimalia gen. nov., that contains the new species Massiliimalia massiliensis gen. nov., sp. nov., and Massiliimalia timonensis gen. nov., sp. nov. Strains Marseille-P2963T (= CSUR P2963 = DSM 106837) and Marseille-P3753T (= CSUR P3753 = CCUG 71632) are their type strains, respectively. |
| اللغة: | English |
| تدمد: | 0003-6072 1572-9699 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7046ce21782f5a401926dfa9d4c4d14eTest https://hal-amu.archives-ouvertes.fr/hal-02202099Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....7046ce21782f5a401926dfa9d4c4d14e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00036072 15729699 |
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