Pancreas preservation fluid microbial contamination is associated with poor islet isolation outcomes - a multi-centre cohort study
| العنوان: | Pancreas preservation fluid microbial contamination is associated with poor islet isolation outcomes - a multi-centre cohort study |
|---|---|
| المؤلفون: | Pierre-Yves Benhamou, Fanny Buron, Yannick D. Muller, Anne Wojtusciszyn, Raphael P. H. Meier, Thierry Berney, Diego O. Andrey, Benoît Bédat, Christian van Delden, Domenico Bosco, Nadja Niclauss, Sophie Borot, Pamela Sun, Nadine Pernin, Sandrine Demuylder-Mischler |
| المساهمون: | Hôpitaux Universitaires de Genève (HUG), University of California [San Francisco] (UCSF), University of California, Department of Surgery, University of Geneva [Switzerland], Institut de mécanique des fluides de Toulouse (IMFT), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Service de Diabétologie - Endocrinologie [CHRU Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Centre Hospitalier Universitaire [Grenoble] (CHU), Laboratory of Fundamental and Applied Bioenergetics = Laboratoire de bioénergétique fondamentale et appliquée (LBFA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL), Université de Strasbourg (UNISTRA), Centre médical universitaire de Genève (CMU) |
| المصدر: | Transplant International Transplant International, Wiley, 2018, 31 (8), pp.917-929. ⟨10.1111/tri.13159⟩ Transplant International (2018) BASE-Bielefeld Academic Search Engine |
| بيانات النشر: | HAL CCSD, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Adult, Male, endocrine system, Adolescent, endocrine system diseases, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Organ Preservation Solutions, Islets of Langerhans Transplantation, Type 1 diabetes mellitus, 030230 surgery, Microbial contamination, medicine.disease_cause, Cold Ischemia Time, Cohort Studies, Andrology, Islets of Langerhans, Young Adult, 03 medical and health sciences, 0302 clinical medicine, medicine, Humans, Islet transplantation, Child, ComputingMilieux_MISCELLANEOUS, Aged, ddc:616, geography, Transplantation, Islet isolation, geography.geographical_feature_category, ddc:617, business.industry, Streptococcus, Middle Aged, Contamination, Islet, Autotransplantation, 3. Good health, medicine.anatomical_structure, Female, 030211 gastroenterology & hepatology, Drug Contamination, Pancreas, business, Staphylococcus, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| الوصف: | The microbiological safety of islet preparations is paramount. Preservation medium contamination is frequent, and its impact on islet yield and function remains unclear. Microbiological samples collected during islet isolations from 2006 to 2016 were analyzed and correlated to isolation and allo- and autotransplantation outcomes. Microbial contamination of preservation medium was found in 64.4% of processed donor pancreases (291/452). We identified 464 microorganisms including Staphylococcus (253/464, 54.5%), Streptococcus (31/464, 6.7%), and Candida species (25/464, 5.4%). Microbial contamination was associated with longer warm and cold ischemia times and lower numbers of postpurification islet equivalents, purity, transplant rate, and stimulation index (all P < 0.05). Six percent of the preparations accepted for transplantation showed microbial contamination after isolation (12/200); 9 of 12 were Candida species. Six patients were transplanted with a sample with late microbial growth discovered after the infusion. Insulin independence rate was not affected. This risk of transplanting a contaminated islets preparation was reduced by half following the implementation of an additional sampling after 24 h of islet culture. Pancreas preservation fluid microbial contamination is associated with lower transplant rate and poorer in vitro function, but not with changes in graft survival. Culture medium testing 1 day after isolation reduces the risk of incidental transplantation with contaminated islets. |
| اللغة: | English |
| تدمد: | 0934-0874 1432-2277 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67d03114427174882e16f785b885254cTest https://hal.univ-grenoble-alpes.fr/hal-01930619Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....67d03114427174882e16f785b885254c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09340874 14322277 |
|---|