المؤلفون: Moreno-Sabater, Alicia, Sterlin, Delphine, Imamovic, Lejla, Bon, Fabienne, Normand, Anne-Cecile, Gonnin, Cecile, Gazzano, Marianne, Bensalah, Merieme, Dorgham, Karim, Ben Salah, Elyes, Acherar, Aniss, Parizot, Christophe, Rigourd, Virginie, Begue, Hervé, Dalle, Frederic, Bachmeyer, Claude, Hennequin, Christophe, Yssel, Hans, Malphettes, Marion, Fieschi, Claire, Fadlallah, Jehane, Gorochov, Guy

المساهمون: Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Procédés Alimentaires et Microbiologiques Dijon (PAM), Université de Bourgogne (UB)-Université Bourgogne Franche-Comté COMUE (UBFC)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), CHU Pitié-Salpêtrière AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Tenon AP-HP, Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)

المصدر: ISSN: 0091-6749 ; Journal of Allergy and Clinical Immunology ; https://hal.science/hal-04099050Test ; Journal of Allergy and Clinical Immunology, inPress, ⟨10.1016/j.jaci.2023.03.033⟩.

مصطلحات موضوعية: Mycobiota, homeostatic IgA, IgA deficiency, IgM, Candida albicans, [SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology

الوصف: International audience ; Background: Secretory IgA interacts with commensal bacteria, but its impact on human mycobiota ecology has not been widely explored. In particular, it remains unknown whether human IgA-deficiency is associated with gut fungal dysbiosis.Objectives: Our goal was to study the impact of IgA on gut mycobiota ecology.Methods: The Fungi-flow method was used to characterize fecal, systemic, and maternal IgA, IgM and IgG responses against 14 representative fungal strains (yeast/spores or hyphae forms) in healthy donors (HD, n= 34, 31 and 20, respectively), and also to compare gut mycobiota opsonization by secretory antibodies in HD (n=28) and patients with selective IgA deficiency (SIgAd n=12). Stool mycobiota composition was determined by ITS gene sequencing in HD (n= 23) and SIgAd (n=17). Circulating CD4+ T cell cytokine secretion profiles were determined by intracellular staining. Impact of secretory IgA, purified from breast milk (n=9), on candida growth and intestinal Caco-2 cell invasion was tested in vitro.Results: Homeostatic IgA binds commensal fungi with a body fluid-selective pattern of recognition. In SIgAd patients, fungal gut ecology is preserved by compensatory IgM binding to commensal fungi. Gut Candida albicans overgrowth nevertheless occurs in this condition, but only in clinically symptomatic patients with decreased Th17/22 T cell responses. Indeed, secretory IgA can reduce in vitro budding and invasion of intestinal cells by C. albicans, and therefore exert control on this pathobiont.Conclusion: IgA has a selective impact on C. albicans ecology to preserve fungal-host mutualism.

العلاقة: hal-04099050; https://hal.science/hal-04099050Test; https://hal.science/hal-04099050/documentTest; https://hal.science/hal-04099050/file/JACI-D-22-01348_Revised_unmarked%20copie.pdfTest

الإتاحة: https://doi.org/10.1016/j.jaci.2023.03.033Test
https://hal.science/hal-04099050Test
https://hal.science/hal-04099050/documentTest
https://hal.science/hal-04099050/file/JACI-D-22-01348_Revised_unmarked%20copie.pdfTest

المؤلفون: Moreno-Sabater, Alicia, Sterlin, Delphine, Imamovic, Lejla, Bon, Fabienne, Normand, Anne-Cecile, Gonnin, Cecile, Gazzano, Marianne, Bensalah, Merieme, Dorgham, Karim, Ben Salah, Elyes, Acherar, Aniss, Parizot, Christophe, Rigourd, Virginie, Begue, Hervé, Dalle, Frederic, Bachmeyer, Claude, Hennequin, Christophe, Yssel, Hans, Malphettes, Marion, Fieschi, Claire, Fadlallah, Jehane, Gorochov, Guy

المساهمون: Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Procédés Alimentaires et Microbiologiques Dijon (PAM), Université de Bourgogne (UB)-Université Bourgogne Franche-Comté COMUE (UBFC)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), CHU Pitié-Salpêtrière AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Tenon AP-HP, Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)

المصدر: ISSN: 0091-6749 ; Journal of Allergy and Clinical Immunology ; https://hal.science/hal-04099050Test ; Journal of Allergy and Clinical Immunology, inPress, ⟨10.1016/j.jaci.2023.03.033⟩.

مصطلحات موضوعية: Mycobiota, homeostatic IgA, IgA deficiency, IgM, Candida albicans, [SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology

الوصف: International audience ; Background: Secretory IgA interacts with commensal bacteria, but its impact on human mycobiota ecology has not been widely explored. In particular, it remains unknown whether human IgA-deficiency is associated with gut fungal dysbiosis.Objectives: Our goal was to study the impact of IgA on gut mycobiota ecology.Methods: The Fungi-flow method was used to characterize fecal, systemic, and maternal IgA, IgM and IgG responses against 14 representative fungal strains (yeast/spores or hyphae forms) in healthy donors (HD, n= 34, 31 and 20, respectively), and also to compare gut mycobiota opsonization by secretory antibodies in HD (n=28) and patients with selective IgA deficiency (SIgAd n=12). Stool mycobiota composition was determined by ITS gene sequencing in HD (n= 23) and SIgAd (n=17). Circulating CD4+ T cell cytokine secretion profiles were determined by intracellular staining. Impact of secretory IgA, purified from breast milk (n=9), on candida growth and intestinal Caco-2 cell invasion was tested in vitro.Results: Homeostatic IgA binds commensal fungi with a body fluid-selective pattern of recognition. In SIgAd patients, fungal gut ecology is preserved by compensatory IgM binding to commensal fungi. Gut Candida albicans overgrowth nevertheless occurs in this condition, but only in clinically symptomatic patients with decreased Th17/22 T cell responses. Indeed, secretory IgA can reduce in vitro budding and invasion of intestinal cells by C. albicans, and therefore exert control on this pathobiont.Conclusion: IgA has a selective impact on C. albicans ecology to preserve fungal-host mutualism.

العلاقة: hal-04099050; https://hal.science/hal-04099050Test; https://hal.science/hal-04099050/documentTest; https://hal.science/hal-04099050/file/JACI-D-22-01348_Revised_unmarked%20copie.pdfTest

الإتاحة: https://doi.org/10.1016/j.jaci.2023.03.033Test
https://hal.science/hal-04099050Test
https://hal.science/hal-04099050/documentTest
https://hal.science/hal-04099050/file/JACI-D-22-01348_Revised_unmarked%20copie.pdfTest

المؤلفون: Moreno-Sabater, Alicia, Sterlin, Delphine, Imamovic, Lejla, Bon, Fabienne, Normand, Anne-Cecile, Gonnin, Cecile, Gazzano, Marianne, Bensalah, Merieme, Dorgham, Karim, Ben Salah, Elyes, Acherar, Aniss, Parizot, Christophe, Rigourd, Virginie, Begue, Hervé, Dalle, Frederic, Bachmeyer, Claude, Hennequin, Christophe, Yssel, Hans, Malphettes, Marion, Fieschi, Claire, Fadlallah, Jehane, Gorochov, Guy

المساهمون: Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Procédés Alimentaires et Microbiologiques Dijon (PAM), Université de Bourgogne (UB)-Université Bourgogne Franche-Comté COMUE (UBFC)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), CHU Pitié-Salpêtrière AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Tenon AP-HP, Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)

المصدر: ISSN: 0091-6749 ; Journal of Allergy and Clinical Immunology ; https://hal.science/hal-04099050Test ; Journal of Allergy and Clinical Immunology, In press, ⟨10.1016/j.jaci.2023.03.033⟩.

مصطلحات موضوعية: Mycobiota, homeostatic IgA, IgA deficiency, IgM, Candida albicans, [SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology

الوصف: International audience ; Background: Secretory IgA interacts with commensal bacteria, but its impact on human mycobiota ecology has not been widely explored. In particular, it remains unknown whether human IgA-deficiency is associated with gut fungal dysbiosis.Objectives: Our goal was to study the impact of IgA on gut mycobiota ecology.Methods: The Fungi-flow method was used to characterize fecal, systemic, and maternal IgA, IgM and IgG responses against 14 representative fungal strains (yeast/spores or hyphae forms) in healthy donors (HD, n= 34, 31 and 20, respectively), and also to compare gut mycobiota opsonization by secretory antibodies in HD (n=28) and patients with selective IgA deficiency (SIgAd n=12). Stool mycobiota composition was determined by ITS gene sequencing in HD (n= 23) and SIgAd (n=17). Circulating CD4+ T cell cytokine secretion profiles were determined by intracellular staining. Impact of secretory IgA, purified from breast milk (n=9), on candida growth and intestinal Caco-2 cell invasion was tested in vitro.Results: Homeostatic IgA binds commensal fungi with a body fluid-selective pattern of recognition. In SIgAd patients, fungal gut ecology is preserved by compensatory IgM binding to commensal fungi. Gut Candida albicans overgrowth nevertheless occurs in this condition, but only in clinically symptomatic patients with decreased Th17/22 T cell responses. Indeed, secretory IgA can reduce in vitro budding and invasion of intestinal cells by C. albicans, and therefore exert control on this pathobiont.Conclusion: IgA has a selective impact on C. albicans ecology to preserve fungal-host mutualism.

العلاقة: hal-04099050; https://hal.science/hal-04099050Test; https://hal.science/hal-04099050/documentTest; https://hal.science/hal-04099050/file/JACI-D-22-01348_Revised_unmarked%20copie.pdfTest

الإتاحة: https://doi.org/10.1016/j.jaci.2023.03.033Test
https://hal.science/hal-04099050Test
https://hal.science/hal-04099050/documentTest
https://hal.science/hal-04099050/file/JACI-D-22-01348_Revised_unmarked%20copie.pdfTest

المؤلفون: Moreno-Sabater, Alicia, Sterlin, Delphine, Imamovic, Lejla, Bon, Fabienne, Normand, Anne-Cecile, Gonnin, Cecile, Gazzano, Marianne, Bensalah, Merieme, Dorgham, Karim, Ben Salah, Elyes, Acherar, Aniss, Parizot, Christophe, Rigourd, Virginie, Begue, Hervé, Dalle, Frederic, Bachmeyer, Claude, Hennequin, Christophe, Yssel, Hans, Malphettes, Marion, Fieschi, Claire, Fadlallah, Jehane, Gorochov, Guy

المساهمون: Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Procédés Alimentaires et Microbiologiques Dijon (PAM), Université de Bourgogne (UB)-Université Bourgogne Franche-Comté COMUE (UBFC)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), CHU Pitié-Salpêtrière AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Tenon AP-HP, Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)

المصدر: ISSN: 0091-6749 ; Journal of Allergy and Clinical Immunology ; https://hal.science/hal-04099050Test ; Journal of Allergy and Clinical Immunology, In press, ⟨10.1016/j.jaci.2023.03.033⟩.

مصطلحات موضوعية: Mycobiota, homeostatic IgA, IgA deficiency, IgM, Candida albicans, [SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology

الوصف: International audience ; Background: Secretory IgA interacts with commensal bacteria, but its impact on human mycobiota ecology has not been widely explored. In particular, it remains unknown whether human IgA-deficiency is associated with gut fungal dysbiosis.Objectives: Our goal was to study the impact of IgA on gut mycobiota ecology.Methods: The Fungi-flow method was used to characterize fecal, systemic, and maternal IgA, IgM and IgG responses against 14 representative fungal strains (yeast/spores or hyphae forms) in healthy donors (HD, n= 34, 31 and 20, respectively), and also to compare gut mycobiota opsonization by secretory antibodies in HD (n=28) and patients with selective IgA deficiency (SIgAd n=12). Stool mycobiota composition was determined by ITS gene sequencing in HD (n= 23) and SIgAd (n=17). Circulating CD4+ T cell cytokine secretion profiles were determined by intracellular staining. Impact of secretory IgA, purified from breast milk (n=9), on candida growth and intestinal Caco-2 cell invasion was tested in vitro.Results: Homeostatic IgA binds commensal fungi with a body fluid-selective pattern of recognition. In SIgAd patients, fungal gut ecology is preserved by compensatory IgM binding to commensal fungi. Gut Candida albicans overgrowth nevertheless occurs in this condition, but only in clinically symptomatic patients with decreased Th17/22 T cell responses. Indeed, secretory IgA can reduce in vitro budding and invasion of intestinal cells by C. albicans, and therefore exert control on this pathobiont.Conclusion: IgA has a selective impact on C. albicans ecology to preserve fungal-host mutualism.

العلاقة: hal-04099050; https://hal.science/hal-04099050Test; https://hal.science/hal-04099050/documentTest; https://hal.science/hal-04099050/file/JACI-D-22-01348_Revised_unmarked%20copie.pdfTest

الإتاحة: https://doi.org/10.1016/j.jaci.2023.03.033Test
https://hal.science/hal-04099050Test
https://hal.science/hal-04099050/documentTest
https://hal.science/hal-04099050/file/JACI-D-22-01348_Revised_unmarked%20copie.pdfTest