Efficient Transovarial Transmission of Babesia Spp. in Rhipicephalus microplus Ticks Fed on Water Buffalo (Bubalus bubalis)
| العنوان: | Efficient Transovarial Transmission of Babesia Spp. in Rhipicephalus microplus Ticks Fed on Water Buffalo (Bubalus bubalis) |
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| المؤلفون: | OBREGÓN, D., CORONA-GONZÁLEZ, B., DÍAZ-SÁNCHEZ, A. A., ARMAS, Y., ROQUE, E., OLIVEIRA, M. C. de S., CABEZAS-CRUZ, A. |
| المساهمون: | University of Guelph, School of Environmental Sciences, Brazilian National Council for Scientific and Technological Development, Brazil CNPq-474648/210-9Empresa Brasileira de Pesquisa Agropecuaria (Embrapa Pecuaria Sudeste) National Priority Program for Animal and Plant Health, Ministry of Agriculture MINAGRIP131LH003007, Dasiel Obregón, University of Guelph, Belkis Corona-González, CENSA, Adrian Alberto Díaz-Sánchez, CENSA, Yasmani Armas, Universidad Agraria de La Habana, Eugenio Roque, Universidad Agraria de La Habana, MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE, Alejandro Cabezas-Cruz, Université Paris-Est. |
| المصدر: | Pathogens Pathogens, MDPI, 2020, 9 (4), pp.280. ⟨10.3390/pathogens9040280⟩ Pathogens 2020 Pathogens 2020, 2020, 9, ⟨10.3390/pathogens9040280⟩ Pathogens, Vol 9, Iss 280, p 280 (2020) Volume 9 Issue 4 Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| بيانات النشر: | HAL CCSD, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, animal diseases, [SDV]Life Sciences [q-bio], lcsh:R, fungi, reproductive efficiency, lcsh:Medicine, food and beverages, Babesia, QPCR, ticks, qPCR, Ticks, Reproductive efficiency, cattle, parasitic diseases, Cattle, water buffalo, Water buffalo, geographic locations |
| الوصف: | Water buffaloes can be infected by tick-borne pathogens (TBPs) in endemic areas where cattle and buffalo coexist. Among TBPs affecting buffaloes is the Apicomplexan hemoparasites Babesia bovis and B. bigemina, transmitted by Rhipicephalus microplus ticks. However, little empirical evidence exists on whether buffalo can support TBPs&rsquo infection and transmission. A cohort study was designed to measure the infestation levels of R. microplus in buffaloes as well as the ability of buffalo-fed ticks to transmit B. bovis and B. bigemina to their offspring. Tick infestation of different life stages was quantified in cattle and buffalo kept in field conditions in western Cuba. Engorged adult female ticks were allowed to lay eggs in controlled conditions of humidity and temperature, and reproductive parameters were measured and analyzed. Hosts and tick larvae were tested for the presence of Babesia spp. using species-specific qPCR assays. Tick infestation was not observed in adult buffaloes. However, buffalo and cattle calves were equally infested, although the larval survival rate was higher in cattle calves than in buffalo calves. All larval pools (31) obtained from the adult female ticks were positive for B. bovis, whereas only 68% (21/31) was positive for B. bigemina. Among the 10 larval pools negative for B. bigemina, three proceeded from adult females fed on Babesia-negative buffaloes. The other seven pools were from Babesia-positive animals, three from cattle and four from buffalo calves. Babesia infection levels in tick larvae, quantified by qPCR, were similar in female ticks fed on buffalo and bovine calves. We conclude that water buffalo can sustain tick vector populations and support Babesia infection in levels high enough as to be infective for ticks. Our results also validated the hypothesis that adult female ticks fed on buffalo can transmit the pathogens B. bovis and B. bigemina to their offspring. Nevertheless, further laboratory studies are needed to address the question of whether the transovarial transmission of Babesia occurs in the following settings: (1) When adult females are infected previous to the feeding on the buffalo or/and (2) when the adult females acquire the infection while feeding on the buffalo. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2076-0817 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::f54d74e74d27b3b5383cac4422fae8e4Test https://hal.inrae.fr/hal-02957635Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.dedup.wf.001..f54d74e74d27b3b5383cac4422fae8e4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20760817 |
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