دورية أكاديمية
Dissection of Pelvic Autonomic Ganglia and Associated Nerves in Male and Female Rats
| العنوان: | Dissection of Pelvic Autonomic Ganglia and Associated Nerves in Male and Female Rats |
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| المؤلفون: | Bertrand, Martin, Keast, Janet |
| المساهمون: | Department of Anatomy & Neuroscience Melbourne, School of Biomedical Sciences Melbourne, Faculty of Medicine, Dentistry and Health Sciences Melbourne, University of Melbourne-University of Melbourne-Faculty of Medicine, Dentistry and Health Sciences Melbourne, University of Melbourne-University of Melbourne, Centre Hospitalier Universitaire de Nîmes (CHU Nîmes) |
| المصدر: | ISSN: 1940-087X. |
| بيانات النشر: | HAL CCSD JoVE |
| سنة النشر: | 2020 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | MESH: Anatomic Landmarks, Animals, Axons / physiology, Dissection, Female, Ganglia, Autonomic / surgery, MESH: Ganglia, Sympathetic / surgery, Male, Nerve Tissue / surgery, Pelvis / innervation, Rats, Sprague-Dawley, [SDV.BA]Life Sciences [q-bio]/Animal biology, [SCCO.NEUR]Cognitive science/Neuroscience, [SDV.MHEP.AHA]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] |
| الوصف: | International audience ; The bilateral major pelvic ganglia (MPG; synonym, pelvic ganglia) are the primary source of postganglionic sympathetic and parasympathetic neurons innervating pelvic organs of rodents; the functionally equivalent structure in humans is the inferior hypogastric plexus. The major pelvic ganglia also provide the route by which lumbar and sacral sensory axons reach the pelvic organs. These complex, mixed ganglia can prove challenging to identify and dissect for further experimental study of normal autonomic mechanisms or to establish preclinical models of disease, injury or visceral pain. Here we describe a protocol to access and visualize these ganglia and their associated nerve tracts. We provide this protocol with schematics for both male and female rats, as the ganglion size and landmarks for identification differ between sexes. The protocol describes removal of the ganglion for in vitro studies, but this method can be integrated into a surgical recovery protocol for experimental interventions (e.g., nerve crush, nerve resection) or for mapping neuronal circuits (e.g., by microinjection of neural tracers). We also demonstrate the primary structures of the ganglion and its associated nerves immediately following dissection and following immunohistochemical staining. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-03349312; https://hal.umontpellier.fr/hal-03349312Test |
| DOI: | 10.3791/60904 |
| الإتاحة: | https://doi.org/10.3791/60904Test https://hal.umontpellier.fr/hal-03349312Test |
| حقوق: | http://creativecommons.org/licenses/byTest/ |
| رقم الانضمام: | edsbas.B5F684F7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3791/60904 |
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