التفاصيل البيبلوغرافية
| العنوان: |
Investigation of the Metabolism of Astragaloside IV in a Puromycin-Damaged Rat Model by UPLC-Q-TOF-MS/MS Analysis. |
| المؤلفون: |
Zhang, Bing, Huang, Shiying, Liu, Zhuoting, Liu, Xinhui, Jiang, Zilan, Chen, Jianping, Zeng, Youjia |
| المصدر: |
Planta Medica; Feb2024, Vol. 90 Issue 2, p154-165, 12p |
| مصطلحات موضوعية: |
FECAL analysis, BIOLOGICAL models, IN vivo studies, LIQUID chromatography-mass spectrometry, ANIMAL experimentation, METABOLISM, MANN Whitney U Test, KIDNEY diseases, RATS, DESCRIPTIVE statistics, ASTRAGALUS (Plants), MOLECULAR structure, DATA analysis software, ENZYME inhibitors, METABOLITES |
| مستخلص: |
Astragaloside IV (AS-IV) has been shown to provide renal protection in various kidney injury models. However, the metabolic profile variation of AS-IV in pathological models in vivo is not well established. This study aims to explore the metabolic pathway of AS-IV in vivo in the classical puromycin aminonucleoside (PAN)-induced kidney injury in a rat model. Twelve Wistar rats were randomly divided into the AS-IV (CA) and the PAN+AS-IV (PA) treatment groups. PAN was injected by a single tail intravenous (i. v.) injection at 5 mg/100 g body weight, and AS-IV was administered intragastrically (i. g.) at 40 mg/kg for 10 days. Fecal samples of these rats were collected, and metabolites of AS-IV were detected by ultra-performance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) to explore the AS-IV metabolic pathway. The metabolic differences between the AS-IV and PAN+AS-IV groups were compared. A total of 25 metabolites were detected, and deglycosylation, deoxygenation, and methyl oxidation were found to be the main metabolic pathways of AS-IV in vivo. The abundance of most of these metabolites in the PAN+AS-IV group was lower than that in the AS-IV treatment group, and differences for seven of them were statistically significant. Our study indicates that AS-IV metabolism is affected in the PAN-induced kidney injury rat model. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
