Heat Shock Protein 47 (HSP47) Antisense Oligonucleotides Reduce Cardiac Remodeling and Improve Cardiac Function in a Rat Model of Myocardial Infarction
| العنوان: | Heat Shock Protein 47 (HSP47) Antisense Oligonucleotides Reduce Cardiac Remodeling and Improve Cardiac Function in a Rat Model of Myocardial Infarction |
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| المؤلفون: | C. Shingu, S Matumoto, T Noguchi, Akira Hasegawa, H. Iwasaka, Satoshi Hagiwara |
| المصدر: | The Thoracic and Cardiovascular Surgeon. 59:386-392 |
| بيانات النشر: | Georg Thieme Verlag KG, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Male, Pulmonary and Respiratory Medicine, Cardiac function curve, medicine.medical_specialty, Time Factors, animal structures, Blotting, Western, Myocardial Infarction, Ventricular Function, Left, Ventricular Dysfunction, Left, Internal medicine, Ventricular Pressure, medicine, Animals, Myocardial infarction, Rats, Wistar, Ventricular remodeling, HSP47 Heat-Shock Proteins, Heat shock protein 47, Ventricular Remodeling, biology, business.industry, Myocardium, Genetic Therapy, Oligonucleotides, Antisense, medicine.disease, Immunohistochemistry, Rats, Disease Models, Animal, medicine.anatomical_structure, Ventricle, Injections, Intravenous, embryonic structures, cardiovascular system, Ventricular pressure, biology.protein, Cardiology, Surgery, Collagen, Cardiology and Cardiovascular Medicine, business, Ligation, Artery |
| الوصف: | Background Cardiac remodeling after acute myocardial infarction is regulated by components of the extracellular matrix. The 47 kD heat shock protein 47 (HSP47) is a collagen-specific molecular chaperone that plays a major role during procollagen processing and/or secretion. Objective The purpose of the study was to determine whether HSP47 inhibition can mitigate ligated left anterior descending (LAD) coronary artery-induced myocardial infarction in rats. Methods Rats were randomly divided into four experimental groups and subjected to the following treatments: 1) intravenous (IV) administration of saline; 2) ligation of the LAD coronary artery; 3) ligation of the LAD coronary artery + IV administration of HSP47 antisense oligonucleotides; or 4) IV administration of HSP47 antisense oligonucleotides. We investigated cardiac histopathology, performed immunoblot and immunohistochemical analyses, and examined cardiac function. Results Rats with ligated LAD coronary artery experienced upregulation of HSP47 expression, remodeling of the left ventricle, and cardiac dysfunction. In contrast, rats with ligated LAD coronary artery treated with HSP47 antisense oligonucleotides had significantly reduced HSP47 expression, cardiac remodeling, and improved cardiac function. Intravenous (IV) administration of HSP47 antisense oligonucleotides alone had no effect on cardiac morphology. Conclusion The data strongly support the idea that changes in the extracellular matrix and its components are important determinants of cardiac remodeling after myocardial infarction. |
| تدمد: | 1439-1902 0171-6425 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2ec7b6a054d19557aa3c74980fda536Test https://doi.org/10.1055/s-0030-1250658Test |
| رقم الانضمام: | edsair.doi.dedup.....c2ec7b6a054d19557aa3c74980fda536 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14391902 01716425 |
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