المؤلفون: Hui-Fen Liao, Y.-J. Chen, Jasson Chiang, L.-M. Chiang, L.-Y. Lai, Y.-Y. Chen

المصدر: International Journal of Sports Medicine. 28:798-803

مصطلحات موضوعية: medicine.medical_specialty, Time Factors, Genes, MHC Class II, Antigen-Presenting Cells, Physical Therapy, Sports Therapy and Rehabilitation, Kidney, Rats, Sprague-Dawley, Immune system, Bone Marrow, Endurance training, Internal medicine, medicine, Animals, Orthopedics and Sports Medicine, Antigen-presenting cell, Interleukin 4, MHC class II, biology, business.industry, Body Weight, Granulocyte-Macrophage Colony-Stimulating Factor, Interleukin, Dendritic Cells, Interleukin-12, Rats, Endocrinology, Granulocyte macrophage colony-stimulating factor, Liver, Immunology, Physical Endurance, biology.protein, Interleukin 12, Interleukin-4, Lymphocyte Culture Test, Mixed, business, medicine.drug

الوصف: A periodized endurance training is able to adapt physical and psychological needs and increase exercise performance. Dendritic cells (DCs) play a key role in regulation of the immune response. In the present study, we trained Sprague-Dawley (SD) rats for five weeks using a progressive endurance protocol with the aim of measuring the effect on myeloid DC differentiation and maturation. Rats were divided into a non-exercise group (NEG) and an exercise group (EG). Bone marrow cells were isolated from these rats after training and cultured in the presence of granulocyte/monocyte-colony stimulatory factor (GM-CSF) and interleukin (IL)-4, and the resultant immature DCs were triggered with lipopolysaccharide to mature. DCs were collected and the main characteristics of DCs were assessed. The recovery rate and the expression of major histocompatibility complex (MHC) class II molecules for DC collected from EG was markedly greater than NEG. The function of DCs from EG to trigger a mixed leukocyte reaction and IL-12 production was higher than NEG. There was no liver and renal toxicity observed in all rats. Changes in food/water consumption and body weight increase between the groups were normal for the conditions. This study demonstrated that periodized endurance training is able to modulate DC development and shift them towards a more mature state.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00108a8b82bf3b979e52551f1fd40756Test
https://doi.org/10.1055/s-2007-964914Test

المؤلفون: Hans K. Schackert, Eberhard Kuhlisch, A. Steinmetz, Weilemann F, Gabriele Schackert, Matthias Kirsch, A. Buttler, S. Kunze

المصدر: Zentralblatt für Neurochirurgie. 64:65-70

مصطلحات موضوعية: Hemagglutinin (influenza), Hemagglutinin Glycoproteins, Influenza Virus, Virus, Mice, Cecum, Antigen, Tumor Cells, Cultured, medicine, Animals, Interleukin 4, Mice, Inbred BALB C, Lung, biology, Brain Neoplasms, business.industry, medicine.disease, Survival Analysis, medicine.anatomical_structure, Injections, Intra-Arterial, Immunization, Colonic Neoplasms, Immunology, Cancer research, biology.protein, Female, Surgery, Interleukin-4, Neurology (clinical), business, Carotid Artery, Internal, Neoplasm Transplantation, Brain metastasis

الوصف: INTRODUCTION Expression of hemagglutinin antigen of influenza virus (HA) by the murine colon carcinoma cell line (CT-26) produces systemic immunization against tumor challenges in the cecum, liver and lungs but not in the brain of BALB/c-mice. Immunization with IL-4 expressing CT-26 cells inhibits lung metastases formation. The purpose of our study was to examine the effects of HA or IL-4 expression on brain metastases formation. METHODS Using selective internal carotid artery injections, brain metastases formation of HA or IL-4 expressing CT-26 cells with and without subcutaneous pre-immunization was evaluated in Balb/c mice. RESULTS Systemic pre-immunization with HA or IL-4 expressing tumor cells cannot protect against brain metastases, while the local, intracerebral expression of HA or IL-4 inhibits the growth of hematogenous brain metastases. CONCLUSION Pre-immunization with HA or IL-4 expressing tumor cells did produce systemic immunity against liver and lung metastases but not against brain metastases. Local, intracerebral expression of HA or IL-4 prevents from cerebral metastases formation in an animal model.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e54b3efa6b907e248b84672245416e77Test
https://doi.org/10.1055/s-2003-40374Test

المؤلفون: Małgorzata Szelachowska, Adam Kretowski, Ida Kinalska

المصدر: Hormone and Metabolic Research. 30:526-530

مصطلحات موضوعية: medicine.medical_specialty, business.industry, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Biochemistry (medical), Clinical Biochemistry, General Medicine, medicine.disease, Biochemistry, Interleukin 10, Endocrinology, Cytokine, Internal medicine, Diabetes mellitus, Immunology, medicine, First-degree relatives, business, Incubation, Interleukin 4, NOD mice, Whole blood

الوصف: There is mounting evidence that the imbalance between Th1 and Th2 lymphocyte subsets plays a key role in the development of autoimmune diabetes in NOD mice, but it is also possible in humans. The aim of the present study was the estimation of in vitro production of Th1 (INF-gamma, IL-2) and Th2-derived (IL-4, IL-10) cytokines by peripheral blood in ICA and GADA positive first degree relatives of Type-I diabetes patients, since they could represent primary events triggering an immune-mediated islets destruction. The study was performed in 25 subjects at risk of insulin-dependent diabetes and 21 age- and sex-matched healthy controls. Cytokine levels in supernatants of whole blood cultures with PHA (10 microg/ml) were quantified by ELISA. We observed a lower concentration of IL-4 in culture supernatants in ICA and GADA positive relatives as compared with the control group, both at 48 h and at 72 h of incubation. Similarly, in the prediabetic group, lower IL-10 levels at 48 and 72 h of culture were found. We did not observe statistical differences in in vitro production of IL-2 and INF-gamma by peripheral blood in high risk diabetes mellitus subjects and healthy controls. In subjects at increased risk of Type-I diabetes, levels of IL-4 positively correlated with those of IL-10. There were negative correlations between IL-10 concentration after 48 h of incubation and levels of HbA1C. In conclusion our study has shown decreased IL-4 and IL-10 production, but normal secretion of Th1-derived cytokines by peripheral blood of prediabetic humans. This could suggest that the early stage of autoimmune process in Type-I diabetes in humans is associated with decreased function of Th2-cells rather than overactivation of Th1 subset in the peripheral blood.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::a86149adeda9f7265054c8f96a582835Test
https://doi.org/10.1055/s-2007-978926Test

المؤلفون: Murray D. Mitchell, Donald J. Dudley, Michael W. Varner, Cheri Hunter

المصدر: ResearcherID

مصطلحات موضوعية: Adult, medicine.medical_specialty, Amniotic fluid, Preterm labor, medicine.medical_treatment, Chorioamnionitis, Proinflammatory cytokine, Pathogenesis, Obstetric Labor, Premature, Pregnancy, medicine, Humans, Interleukin 4, Obstetrics, business.industry, Obstetrics and Gynecology, Increased amniotic fluid, Amniotic Fluid, medicine.disease, Cytokine, Case-Control Studies, Pediatrics, Perinatology and Child Health, Interleukin-2, Female, Interleukin-4, business

الوصف: Preterm labor associated with intrauterine infection is characterized by increased amniotic fluid concentrations of various proinflammatory cytokines, including interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), IL-6, IL-8, and macrophage inflammatory protein-1alpha (MIP-1alpha). The purpose of this study was to determine if preterm labor in women with clinically evident chorioamnionitis is marked by elevations of the anti-inflammatory cytokine interleukin-4 (IL-4) and the T cell growth factor IL-2. Amniotic fluid samples were obtained from (1) women at term, not in labor (n = 10); (2) women at term, in labor (n = 10); (3) women with preterm contractions but undelivered within 1 week of amniotic fluid collection (n = 10); (4) women with preterm labor and delivery without clinically evident chorioamnionitis (n = 10); (5) women with preterm labor associated with chorioamnionitis (n = 8); and (6) women with preterm labor and delivery without infection matched with patients with chorioamnionitis (n = 8). Amniotic fluid concentrations of IL-4 and IL-2 were determined for each sample with a specific and sensitive enzyme-linked immunoassay. We found that women with infection-associated preterm labor and delivery had significantly higher concentrations of IL-4 when compared to appropriately matched controls (p0.05). Additionally, women with preterm labor and delivery not associated with infection had higher amniotic fluid IL-4 concentrations than women with preterm contractions but no labor (p0.05). Women with term labor had rare modest elevations of amniotic fluid IL-4. No IL-2 was detected in any sample. Our data indicate that amniotic fluid IL-4 is elevated in women with preterm labor and delivery, particularly in association with chorioamnionitis. We suggest that IL-4, although previously considered an anti-inflammatory agent, may have a paradoxical proinflammatory role in the pathogenesis of infection-associated preterm labor.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d41ec9cfa86dcc3743076d271c5cdd5Test
https://doi.org/10.1055/s-2007-994385Test

المؤلفون: L Kolesar, M Sterclova, M Vasakova, R Matej

المصدر: Pneumologie. 66

مصطلحات موضوعية: Pulmonary and Respiratory Medicine, Idiopathic pulmonary fibrosis, Pathology, medicine.medical_specialty, business.industry, medicine, Lung tissue, medicine.disease, business, Gene, Interleukin 4

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::69a515f4213a92588d56d80f41208ab1Test
https://doi.org/10.1055/s-0032-1329823Test

المؤلفون: M Kornamnn, H Fricke, Doris Henne-Bruns, A Formentini, P Braun

المصدر: Zeitschrift für Gastroenterologie. 49

مصطلحات موضوعية: Expression (architecture), business.industry, Gastroenterology, Cancer research, medicine, Cancer, Receptor, medicine.disease, business, Interleukin 4

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::5d4c1750d8e7329780b85b847847db1fTest
https://doi.org/10.1055/s-0031-1285289Test

المؤلفون: Mohammad Ebrahim Azemi, M Ghafoorian Borougerdnia, H Saeidian, Ali Asghar Hemmati, S Yousef Naanaei, F Namjooyan

المصدر: Planta Medica. 77

مصطلحات موضوعية: Pharmacology, biology, business.industry, Organic Chemistry, Pharmaceutical Science, Analytical Chemistry, Astragalus fasciculifolius, Ovalbumin, Complementary and alternative medicine, Immunological Factors, Drug Discovery, Immunology, biology.protein, Molecular Medicine, Medicine, business, Interleukin 4

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::e7b640802cec371853a92ecb7ef6214bTest
https://doi.org/10.1055/s-0031-1282895Test

المؤلفون: C. von Schilling, J. Seissler, Matthias Schott, Joachim Feldkamp, Werner A. Scherbaum

المصدر: Hormone and Metabolic Research. 31:662-664

مصطلحات موضوعية: Male, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Clinical Biochemistry, Parathyroid hormone, Biochemistry, Endocrinology, Immune system, Biomarkers, Tumor, Humans, Medicine, Macrophage, Hypersensitivity, Delayed, Interleukin 4, business.industry, Biochemistry (medical), Dendritic Cells, General Medicine, Immunotherapy, Dendritic cell, Middle Aged, medicine.disease, Carcinoma, Neuroendocrine, Pancreatic Neoplasms, Parathyroid Neoplasms, medicine.anatomical_structure, Parathyroid carcinoma, Parathyroid Hormone, Immunology, Female, business, Pancreas, Immunologic Memory

الوصف: Parathyroid carcinomas and neuroendocrine carcinomas of the pancreas are rare malignancies in humans. Because of their low radio- and chemosensibility, they fail to respond to conventional therapy. We therefore tested a dendritic cell immunotherapy in an attempt to control the tumour growth in two patients. Studies on mice and humans have demonstrated the potent capacity of dendritic cells to induce specific antitumour immunity. Mature dendritic cells were generated from peripheral blood monocytes in the presence of granulocyte/macrophage colony-stimulating factor, interleukin 4 and tumour necrosis factor alpha. Dendritic cells were either loaded with parathyroid hormone (PTH) or with (pancreas) tumour-derived lysate (TL), respectively, and were delivered by subcutaneous injections. All immunizations were well tolerated with no side effects, and were administered on an outpatient basis. After repeated vaccinations, specific in vivo immune response was demonstrated by positive delayed-type hypersensitivity (DTH) toward PTH or TL, demonstrating the efficient generation of antigen-specific memory T-cells. DTH reactivity was accompanied by a significant decrease of tumour markers in both patients. This approach might be generally applicable to other advanced, radio- and chemotherapy-resistant endocrine malignancies.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8100921d4d53c9874d24e4f397d9401Test
https://doi.org/10.1055/s-2007-978817Test

المؤلفون: A. Melms, H. Guo, M. Röcken, R. Weissert, F. Bischof

المصدر: Aktuelle Neurologie. 32

مصطلحات موضوعية: medicine.anatomical_structure, business.industry, Immunology, Central nervous system, medicine, Neurology (clinical), medicine.disease_cause, business, Interleukin 4, Autoimmunity

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::d66d2a471b4797dd6133286fe42f8ab2Test
https://doi.org/10.1055/s-2005-919402Test