دورية أكاديمية
DNA prime and protein boost immunization with innovative polymeric cationic core-shell nanoparticles elicits broad immune response and strongly enhance cellular responses of HIV-1 tat DNA vaccination.
| العنوان: | DNA prime and protein boost immunization with innovative polymeric cationic core-shell nanoparticles elicits broad immune response and strongly enhance cellular responses of HIV-1 tat DNA vaccination. |
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| المؤلفون: | CASTALDELLO, ARIANNA, ALTAVILLA, GIUSEPPE, CAPUTO, ANTONELLA, BROCCA COFANO E, VOLTAN R, TRIULZI C, LAUS M, SPARNACCI K, BALLESTRI M, TONDELLI L, FORTIN C, GAVIOLI R, ENSOLI B |
| المساهمون: | Castaldello, Arianna, BROCCA COFANO, E, Voltan, R, Triulzi, C, Altavilla, Giuseppe, Laus, M, Sparnacci, K, Ballestri, M, Tondelli, L, Fortin, C, Gavioli, R, Ensoli, B, Caputo, Antonella |
| بيانات النشر: | G. POLAND |
| سنة النشر: | 2006 |
| المجموعة: | Padua Research Archive (IRIS - Università degli Studi di Padova) |
| الوصف: | Novel biocompatible core-shell cationic nanoparticles, composed of an inner hard core of poly(methylmethacrylate) (PMMA) and a hydrophilic tentacular shell bearing positively charged groups and poly(ethyleneglycol) chains covalently bound to the core, were prepared by emulsion polymerization and characterized in vitro and in vivo for DNA vaccine applications. The nanoparticles reversibly adsorbed large amounts of DNA, mainly through electrostatic interactions, preserved its functional structure, efficiently delivered it intracellularly, and were not toxic in vitro or in mice. Furthermore, two intramuscular (i.m.) immunizations (4 weeks apart) with a very low dose (1 microg) of the plasmid pCV-tat delivered by these nanoparticles followed by one or two protein boosts induced significant antigen-specific humoral and cellular responses and greatly increased Th1-type T cell responses and CTLs against HIV-1 Tat. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/16781023; info:eu-repo/semantics/altIdentifier/wos/WOS:000239081600001; volume:24; firstpage:5655; lastpage:5669; journal:VACCINE; http://hdl.handle.net/11577/2458995Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-33745284588; http://www.journals.elsevier.com/vaccineTest/ |
| DOI: | 10.1016/j.vaccine.2006.05.058 |
| الإتاحة: | https://doi.org/10.1016/j.vaccine.2006.05.058Test http://hdl.handle.net/11577/2458995Test http://www.journals.elsevier.com/vaccineTest/ |
| رقم الانضمام: | edsbas.9DD823CB |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.vaccine.2006.05.058 |
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