دورية أكاديمية
أعرض في EDS

Hi-Plex2: a simple and robust approach to targeted sequencing-based genetic screening

التفاصيل البيبلوغرافية
العنوان: Hi-Plex2: a simple and robust approach to targeted sequencing-based genetic screening
المؤلفون: Fleur Hammet, Khalid Mahmood, Thomas R Green, Tu Nguyen-Dumont, Melissa C Southey, Daniel D Buchanan, Andrew Lonie, Katherine L Nathanson, Fergus J Couch, Bernard J Pope, Daniel J Park
المصدر: BioTechniques, Vol 67, Iss 3, Pp 118-122 (2019)
بيانات النشر: Future Science Ltd
سنة النشر: 2019
المجموعة: Directory of Open Access Journals: DOAJ Articles
مصطلحات موضوعية: genetic screening, Hi-Plex, multiplex PCR, targeted DNA sequencing, variant detection, Biology (General), QH301-705.5
الوصف: We have previously reported Hi-Plex, a multiplex PCR methodology for building targeted DNA sequencing libraries that offers a low-cost protocol compatible with high-throughput processing. Here, we detail an improved protocol, Hi-Plex2, that more effectively enables the robust construction of small-to-medium panel-size libraries while maintaining low cost, simplicity and accuracy benefits of the Hi-Plex platform. Hi-Plex2 was applied to three panels, comprising 291, 740 and 1193 amplicons, targeting genes associated with risk for breast and/or colon cancer. We show substantial reduction of off-target amplification to enable library construction for small-to-medium-sized design panels not possible using the previous Hi-Plex chemistry.
نوع الوثيقة: article in journal/newspaper
اللغة: English
تدمد: 1940-9818
0736-6205
العلاقة: https://doaj.org/toc/0736-6205Test; https://doaj.org/toc/1940-9818Test; https://doaj.org/article/a1ee4f8f808a45a4bf9a2955912f5cc8Test
DOI: 10.2144/btn-2019-0026
الإتاحة: https://doi.org/10.2144/btn-2019-0026Test
https://doaj.org/article/a1ee4f8f808a45a4bf9a2955912f5cc8Test
رقم الانضمام: edsbas.5F2FE119
قاعدة البيانات: BASE
الوصف
تدمد:19409818
07366205
DOI:10.2144/btn-2019-0026