دورية أكاديمية
Affinity proteomics to study endogenous protein complexes: Pointers, pitfalls, preferences and perspectives
| العنوان: | Affinity proteomics to study endogenous protein complexes: Pointers, pitfalls, preferences and perspectives |
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| المؤلفون: | John LaCava, Kelly R. Molloy, Martin S. Taylor, Michal Domanski, Brian T. Chait, Michael P. Rout |
| المصدر: | BioTechniques, Vol 58, Iss 3, Pp 103-119 (2015) |
| بيانات النشر: | Future Science Ltd |
| سنة النشر: | 2015 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | protein complex, protein purification, affinity, proteomics, interactomics, Biology (General), QH301-705.5 |
| الوصف: | Dissecting and studying cellular systems requires the ability to specifically isolate distinct proteins along with the co-assembled constituents of their associated complexes. Affinity capture techniques leverage high affinity, high specificity reagents to target and capture proteins of interest along with specifically associated proteins from cell extracts. Affinity capture coupled to mass spectrometry (MS)-based proteomic analyses has enabled the isolation and characterization of a wide range of endogenous protein complexes. Here, we outline effective procedures for the affinity capture of protein complexes, highlighting best practices and common pitfalls. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1940-9818 0736-6205 |
| العلاقة: | https://doaj.org/toc/0736-6205Test; https://doaj.org/toc/1940-9818Test; https://doaj.org/article/0ccc91c451e746108840644f6ad31d2dTest |
| DOI: | 10.2144/000114262 |
| الإتاحة: | https://doi.org/10.2144/000114262Test https://doaj.org/article/0ccc91c451e746108840644f6ad31d2dTest |
| رقم الانضمام: | edsbas.4AA89C23 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19409818 07366205 |
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| DOI: | 10.2144/000114262 |