Large-scale implementation of sequential protein and peptide immunoaffinity enrichment LC/nanoLC–MS/MS for human β-nerve growth factor
| العنوان: | Large-scale implementation of sequential protein and peptide immunoaffinity enrichment LC/nanoLC–MS/MS for human β-nerve growth factor |
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| المؤلفون: | Gary A. Schultz, Kathlyn McCardle, Hendrik Neubert |
| المصدر: | Bioanalysis. 8:753-764 |
| بيانات النشر: | Future Science Ltd, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Quality Control, 0301 basic medicine, Clinical Biochemistry, Peptide, Tandem mass spectrometry, 01 natural sciences, Chromatography, Affinity, Analytical Chemistry, 03 medical and health sciences, Tandem Mass Spectrometry, Liquid chromatography–mass spectrometry, Nerve Growth Factor, Humans, Nanotechnology, General Pharmacology, Toxicology and Pharmaceutics, Chromatography, High Pressure Liquid, chemistry.chemical_classification, Chromatography, Chemistry, 010401 analytical chemistry, Nano lc ms ms, General Medicine, Method development, Recombinant Proteins, 0104 chemical sciences, Medical Laboratory Technology, 030104 developmental biology, Nerve growth factor, Biomarker (medicine) |
| الوصف: | Background: A previously described immunoaffinity (IA)-LC-MS/MS assay for human β-nerve growth factor (β-NGF) was implemented to support large-scale sample testing for multiple clinical trials. Methodology & results: The procedure was modified to increase throughput by simultaneous preparation of two 96-well plates and LC duty-cycle reduction. Robustness of the LC method and nano-ESI was ensured during large-scale assay execution by closely monitoring and, if needed, replacing system components prior to failure. Following validation, the assay was used to analyze approximately 19,000 samples from multiple clinical studies over several years. Conclusion: Routine implementation of the β-NGF IA-LC–MS/MS assay supported drug development programs. This optimized assay format now serves as a template for other clinical protein biomarker assays. |
| تدمد: | 1757-6199 1757-6180 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5670a14196874d728c50b1282c673511Test https://doi.org/10.4155/bio-2015-0022Test |
| رقم الانضمام: | edsair.doi.dedup.....5670a14196874d728c50b1282c673511 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17576199 17576180 |
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