دورية أكاديمية
LC-MS/MS simultaneous analysis of allopregnanolone; epiallopregnanolone; pregnanolone; dehydroepiandrosterone and dehydroepiandrosterone 3-sulfate in human plasma
| العنوان: | LC-MS/MS simultaneous analysis of allopregnanolone; epiallopregnanolone; pregnanolone; dehydroepiandrosterone and dehydroepiandrosterone 3-sulfate in human plasma |
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| المؤلفون: | Lionetto, Luana, De Andrã©s, Fernando, Capi, Matilde, Curto, Martina, Sabato, Donata, Simmaco, Maurizio, Bossã1, Paola, Sacchinelli, Eleonora, Orfei, Maria Donata, Piras, Federica, Banaj, Nerisa, Spalletta, Gianfranco |
| المساهمون: | Lionetto, Luana, De Andrã©s, Fernando, Capi, Matilde, Curto, Martina, Sabato, Donata, Simmaco, Maurizio, Bossã1, Paola, Sacchinelli, Eleonora, Orfei, Maria Donata, Piras, Federica, Banaj, Nerisa, Spalletta, Gianfranco |
| بيانات النشر: | Future Medicine Ltd. |
| سنة النشر: | 2017 |
| المجموعة: | Sapienza Università di Roma: CINECA IRIS |
| مصطلحات موضوعية: | allopregnanolone, dehydroepiandrosterone, mass spectrometry, neurosteroid, plasma, pregnanolone, chromatography, liquid, dehydroepiandrosterone sulfate, drug monitoring, human, limit of detection, reproducibility of result, sensitivity and specificity, tandem mass spectrometry, analytical chemistry, pharmacology |
| الوصف: | AIM: Several neuropsychopharmacological properties have been attributed to the 3α-reduced pregnane steroids, allopregnanolone and pregnanolone, as well as to dehydroepiandrosterone sulfate because of their ability to modulate γ-aminobutyric acid (GABAA) receptors in the CNS. In order to understand better their role in several mechanisms in CNS, a new methodology is proposed to monitor these compounds in human plasma. Methodology & results: The analytes were first derivatized with 2-hydrazinopyridine and extracted from plasma using SPE. Then, the compounds were separated and detected by LC-MS/MS. A mobile phase of formic acid (0.1%) in water and methanol through a gradient of composition and a flow rate of 0.3 ml min-1 resulted in good separations of the analytes. Linear responses in wide range of concentrations and LOQs ranging from 10 (dehydroepiandrosterone 3-sulfate) to 40 pg ml-1 (dehydroepiandrosterone) were obtained in <9 min. The method proposed has been validated and then applied to monitor these neurosteroids in plasma samples from ten volunteers. CONCLUSION: For the first time, a straightforward and reliable method for the chromatographic separation of allopregnanolone, epiallopregnanolone and pregnanolone, as well as of dehydroepiandrosterone and dehydroepiandrosterone 3-sulfate was carried out, with optimal accuracy, sensitivity and specificity. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/28207286; info:eu-repo/semantics/altIdentifier/wos/WOS:000395675200004; volume:9; issue:6; firstpage:527; lastpage:539; numberofpages:13; journal:BIOANALYSIS; http://hdl.handle.net/11573/1022112Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85014134288; http://www.future-science.com/loi/bioTest |
| DOI: | 10.4155/bio-2016-0262 |
| الإتاحة: | https://doi.org/10.4155/bio-2016-0262Test http://hdl.handle.net/11573/1022112Test http://www.future-science.com/loi/bioTest |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.E4926291 |
| قاعدة البيانات: | BASE |
| DOI: | 10.4155/bio-2016-0262 |
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