Negative neuronal differentiation of human adipose-derived stem cell clones
| العنوان: | Negative neuronal differentiation of human adipose-derived stem cell clones |
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| المؤلفون: | Enrique Roche, Ana B. Ropero, Juan A. Reig, Agustín F. Fernández, María I. Arribas, Mario F. Fraga, Alfredo Santana |
| المصدر: | Regenerative Medicine. 9:279-293 |
| بيانات النشر: | Future Medicine Ltd, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Adult, Embryology, Cell Culture Techniques, Biomedical Engineering, Adipose tissue, Biology, Real-Time Polymerase Chain Reaction, Gene expression, Humans, Gene, DNA Primers, Electrophoresis, Agar Gel, Neural Plate, Neuroectoderm, Mesenchymal stem cell, Cell Differentiation, Mesenchymal Stem Cells, Promoter, DNA Methylation, Immunohistochemistry, Cell biology, Adult Stem Cells, Adipose Tissue, Immunology, DNA methylation, Female, Adult stem cell |
| الوصف: | Aims: Adipose mesenchymal stem cells are a heterogeneous population. Therefore, the question posed in this study is whether the heterogenic differentiation potential exhibited by the different clones toward mesodermic lineages can be extended to nonmesodermic lineages, such as the neuroectoderm. Materials & methods: Different single cell clones of human adipose mesenchymal stem cells from the same donor were isolated. Neuronal plasticity of the clones was assessed according to the pattern DNA methylation, gene expression and intracellular calcium responses. Results: Under neurogenic culture conditions, clones presented variable expression of neuronal-specific genes, but still expressed osteogenic markers. No calcium response was exhibited in response to KCl incubation. The DNA methylation profile presented a very similar pattern in neuroectoderm gene promoters. Conclusions: Data indicate that there are no significant differences between the undifferentiated and supposedly neuronal-differentiated mesenchymal cells. |
| تدمد: | 1746-076X 1746-0751 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5f712054870a555a553678d18ef4560Test https://doi.org/10.2217/rme.14.11Test |
| رقم الانضمام: | edsair.doi.dedup.....d5f712054870a555a553678d18ef4560 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1746076X 17460751 |
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