دورية أكاديمية
Production, characterization, and application of antibodies against heat-labile type-I toxin for detection of enterotoxigenic Escherichia coli
| العنوان: | Production, characterization, and application of antibodies against heat-labile type-I toxin for detection of enterotoxigenic Escherichia coli |
|---|---|
| المؤلفون: | Caroline A Menezes, Sergio Y Imamura, Luiz R Trabulsi, Antônio Fernandes-Filho, Marina B Martinez, Beatriz EC Guth, Dennys M Girão, Roxane MF Piazza |
| المصدر: | Memorias do Instituto Oswaldo Cruz, Vol 101, Iss 8, Pp 875-880 (2006) |
| بيانات النشر: | Fundação Oswaldo Cruz (FIOCRUZ), 2006. |
| سنة النشر: | 2006 |
| المجموعة: | LCC:Microbiology LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | detection, antibodies, heat-labile toxin, Escherichia coli, Microbiology, QR1-502, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Strains of enterotoxigenic Escherichia coli (ETEC) are responsible for significant rates of morbidity and mortality among children, particularly in developing countries. The majority of clinical and public health laboratories are capable of isolating and identifying Salmonella, Shigella, Campylobacter, and Escherichia coli O157:H7 from stool samples, but ETEC cannot be identified by routine methods. The method most often used to identify ETEC is polymerase chain reaction for heat-stable and heat-labile enterotoxin genes, and subsequent serotyping, but most clinical and public health laboratories do not have the capacity or resources to perform these tests. In this study, polyclonal rabbit and monoclonal mouse IgG2b antibodies against ETEC heat-labile toxin-I (LT) were characterized and the potential applicability of a capture assay was analyzed. IgG-enriched fractions from rabbit polyclonal and the IgG2b monoclonal antibodies recognized LT in a conformational shape and they were excellent tools for detection of LT-producing strains. These findings indicate that the capture immunoassay could be used as a diagnostic assay of ETEC LT-producing strains in routine diagnosis and in epidemiological studies of diarrhea in developing countries as enzyme linked immunosorbent assay techniques remain as effective and economical choice for the detection of specific pathogen antigens in cultures. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0074-0276 1678-8060 |
| العلاقة: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000800009Test; https://doaj.org/toc/0074-0276Test; https://doaj.org/toc/1678-8060Test |
| DOI: | 10.1590/S0074-02762006000800009 |
| الوصول الحر: | https://doaj.org/article/2c82387038e24c1380f48132cfa6577dTest |
| رقم الانضمام: | edsdoj.2c82387038e24c1380f48132cfa6577d |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 00740276 16788060 |
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| DOI: | 10.1590/S0074-02762006000800009 |