دورية أكاديمية
The shift from low to high non-structural protein 1 expression in rotavirus-infected MA-104 cells
| العنوان: | The shift from low to high non-structural protein 1 expression in rotavirus-infected MA-104 cells |
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| المؤلفون: | Laura Martinez-Alvarez, Carolina Pina-Vazquez, Wilbert Zarco, Luis Padilla-Noriega |
| المصدر: | Memorias do Instituto Oswaldo Cruz, Vol 108, Iss 4, Pp 421-428 (2013) |
| بيانات النشر: | Fundação Oswaldo Cruz (FIOCRUZ), 2013. |
| سنة النشر: | 2013 |
| المجموعة: | LCC:Microbiology LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | rotavirus, gene expression, interferons type 1, proteasome endopeptidase complex, ubiquitin protein ligases, Microbiology, QR1-502, Infectious and parasitic diseases, RC109-216 |
| الوصف: | A hallmark of group/species A rotavirus (RVA) replication in MA-104 cells is the logarithmic increase in viral mRNAs that occurs four-12 h post-infection. Viral protein synthesis typically lags closely behind mRNA synthesis but continues after mRNA levels plateau. However, RVA non-structural protein 1 (NSP1) is present at very low levels throughout viral replication despite showing robust protein synthesis. NSP1 has the contrasting properties of being susceptible to proteasomal degradation, but being stabilised against proteasomal degradation by viral proteins and/or viral mRNAs. We aimed to determine the kinetics of the accumulation and intracellular distribution of NSP1 in MA-104 cells infected with rhesus rotavirus (RRV). NSP1 preferentially localises to the perinuclear region of the cytoplasm of infected cells, forming abundant granules that are heterogeneous in size. Late in infection, large NSP1 granules predominate, coincident with a shift from low to high NSP1 expression levels. Our results indicate that rotavirus NSP1 is a late viral protein in MA-104 cells infected with RRV, presumably as a result of altered protein turnover. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1678-8060 0074-0276 |
| العلاقة: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000400421&lng=en&tlng=enTest; https://doaj.org/toc/1678-8060Test |
| DOI: | 10.1590/S0074-0276108042013005 |
| الوصول الحر: | https://doaj.org/article/4cc434c021bd42f1bdc1b9cb0ed62041Test |
| رقم الانضمام: | edsdoj.4cc434c021bd42f1bdc1b9cb0ed62041 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16788060 00740276 |
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| DOI: | 10.1590/S0074-0276108042013005 |