Modelling of epithelial growth, fission and lumen formation during embryonic thyroid development: A combination of computational and experimental approaches
| العنوان: | Modelling of epithelial growth, fission and lumen formation during embryonic thyroid development: A combination of computational and experimental approaches |
|---|---|
| المؤلفون: | Leolo Gonay, Catherine Spourquet, Matthieu Baudoin, Ludovic Lepers, Pascale Lemoine, Alexander G. Fletcher, Emmanuel Hanert, Christophe E. Pierreux |
| المساهمون: | UCL - SST/ELI/ELIE - Environmental Sciences |
| المصدر: | Frontiers in Endocrinology, Vol. 12, no.516 (2021) Frontiers in Endocrinology, Vol 12 (2021) Frontiers in Endocrinology |
| بيانات النشر: | Frontiers Research Foundation, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, vertex model, Angiogenesis, Endocrinology, Diabetes and Metabolism, Organogenesis, Morphogenesis, Thyroid Gland, Embryonic Development, morphogenesis, epithelial, Biology, Diseases of the endocrine glands. Clinical endocrinology, thyroid, modelling, 03 medical and health sciences, Mice, 0302 clinical medicine, Endocrinology, Follicular lumen, medicine, Image Processing, Computer-Assisted, Animals, Computer Simulation, Original Research, Thyroid, Endothelial Cells, Epithelial Cells, Models, Theoretical, Embryo, Mammalian, RC648-665, Embryonic stem cell, Cell biology, 030104 developmental biology, medicine.anatomical_structure, Thyroid function, Endoderm, Developmental biology, 030217 neurology & neurosurgery |
| الوصف: | Organogenesis is the phase of embryonic development leading to the formation of fully functional organs. In the case of the thyroid, organogenesis starts from the endoderm and generates a multitude of closely packed independent spherical follicular units surrounded by a dense network of capillaries. Follicular organisation is unique and essential for thyroid function, i.e. thyroid hormone production. Previous in vivo studies showed that, besides their nutritive function, endothelial cells play a central role during thyroid gland morphogenesis. However, the precise mechanisms and biological parameters controlling the transformation of the multi-layered thyroid epithelial primordium into a multitude of single-layered follicles are mostly unknown. Animal studies used to improve understanding of organogenesis are costly and time-consuming, with recognised limitations. Here, we developed and used a 2-D vertex model of thyroid growth, angiogenesis and folliculogenesis, within the open-source Chaste framework. Our in silico model, based on in vivo images, correctly simulates the differential growth and proliferation of central and peripheral epithelial cells, as well as the morphogen-driven migration of endothelial cells, consistently with our experimental data. Our simulations further showed that reduced epithelial cell adhesion was critical to allow endothelial invasion and fission of the multi-layered epithelial mass. Finally, our model also allowed epithelial cell polarisation and follicular lumen formation by endothelial cell abundance and proximity. Our study illustrates how constant discussion between theoretical and experimental approaches can help us to better understand the roles of cellular movement, adhesion and polarisation during thyroid embryonic development. We anticipate that the use of in silico models like the one we describe can push forward the fields of developmental biology and regenerative medicine. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::803db553557964b0a7ab5e10ad9fd814Test https://hdl.handle.net/2078.1/248144Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....803db553557964b0a7ab5e10ad9fd814 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |