المؤلفون: Vandromme, Camille, Kasprowicz, Angelina, Courseaux, Adeline, Trinel, Dave, Facon, Maud, Putaux, Jean-Luc, D'hulst, Christophe, Wattebled, Fabrice, Spriet, Corentin

المساهمون: Université de Lille, CNRS, Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 UGSF, Unité de Glycobiologie Structurale et Fonctionnelle UGSF, Centre de Recherches sur les Macromolécules Végétales CERMAV

مصطلحات موضوعية: starch, confocal fluorescence imaging, machine learning, Arabidopsis, starch granule morphology, autofluorescence

الوصف: Starch granules that accumulate in the plastids of plants vary in size, shape, phosphate, or protein content according to their botanical origin. Depending on their size, the applications in food and nonfood industries differ. Being able to master starch granule size for a specific plant, without alteration of other characteristics (phosphate content, protein content, etc.), is challenging. The development of a simple and effective screening method to determine the size and shape of starch granules in a plant population is therefore of prime interest. In this study, we propose a new method, NegFluo, that combines negative confocal autofluorescence imaging in leaf and machine learning (ML)-based image analysis. It provides a fast, automated, and easy-to-use pipeline for both in situ starch granule imaging and its morphological analysis. NegFluo was applied to Arabidopsis leaves of wild-type and ss4 mutant plants. We validated its accuracy by comparing morphological quantifications using NegFluo and state-of-the-art methods relying either on starch granule purification or on preparation-intensive electron microscopy combined with manual image analysis. NegFluo thus opens the way to fast in situ analysis of starch granules. ; 10

وصف الملف: application/rdf+xml; charset=utf-8; application/octet-stream

العلاقة: Identification du dialogue moléculaire entre la division du chloroplaste et le métabolisme de l'amidon; Frontiers in Plant Science; Front. Plant Sci.; http://hdl.handle.net/20.500.12210/29592Test

الإتاحة: https://doi.org/20.500.12210/29592Test
https://hdl.handle.net/20.500.12210/29592Test

المؤلفون: Qiaoquan Liu, Cunxu Wei, Pan Hu, Juan Wang, Zichun Chen

المصدر: Frontiers in Plant Science

مصطلحات موضوعية: 0106 biological sciences, Starch, Review, Plant Science, Branching (polymer chemistry), 01 natural sciences, Endosperm, chemistry.chemical_compound, Hydrolysis, high-amylose cereal crop, starch property, 0404 agricultural biotechnology, Amylose, Botany, Food science, starch molecular structure, Starch Branching Enzyme, chemistry.chemical_classification, food and beverages, 04 agricultural and veterinary sciences, 040401 food science, heterogeneous starch granule, Enzyme, chemistry, Amylopectin, starch branching enzyme, 010606 plant biology & botany

الوصف: High-amylose cereal starches provide many health benefits for humans. The inhibition or mutation of starch branching enzyme (SBE) genes is an effective method to develop high-amylose cereal crops. This review summarizes the development of high-amylose cereal crops through the inactivation of one or more SBE isoforms or combination with other genes. This review also reveals the causes of increase in amylose content in high-amylose crops. A series of changes, including amylopectin structure, crystalline structure, thermal properties, and hydrolysis properties, occurs as amylose content increases. The different morphological starch granules nominated as heterogeneous starch granules or differently stained starch granules are detected in high-amylose cereal crops. Detailed studies on four heterogeneous starch granules in high-amylose rice, which is developed by antisense RNA inhibition of SBEI/IIb, indicate that granules with different morphologies possess various molecular structures and physicochemical and functional properties. This variation diversifies their applications in food and non-food industries. However, current knowledge regarding how these heterogeneous starch granules form and why they exhibit regional distribution in endosperm remain largely unknown.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ef057128ad41435bcfc112d4ddb30f8Test

المؤلفون: Robert A. Field, Ellis C. O’Neill

المصدر: Frontiers in Bioengineering and Biotechnology, Vol 3 (2015)
Frontiers in Bioengineering and Biotechnology

مصطلحات موضوعية: Renewable materials, Histology, Starch, Mini Review, lcsh:Biotechnology, phosphorylase, Biomedical Engineering, Bioengineering, Biology, Synthetic biology, chemistry.chemical_compound, lcsh:TP248.13-248.65, Nanotechnology, Metal nanoparticles, Glucans, Liquid crystalline, Bioengineering and Biotechnology, food and beverages, self-assembly, Biochemistry, chemistry, Starch granule, Synthetic Biology, Carbohydrate active enzymes, Biogenesis, Biotechnology

الوصف: Starch makes up more than half of the calories in the human diet and is also a valuable bulk commodity that is used across the food, brewing and distilling, medicines and renewable materials sectors. Despite its importance, our understanding of how plants make starch, and what controls the deposition of this insoluble, polymeric, liquid crystalline material, remains rather limited. Advances are hampered by the challenges inherent in analyzing enzymes that operate across the solid–liquid interface. Glyconanotechnology, in the form of glucan-coated sensor chips and metal nanoparticles, present novel opportunities to address this problem. Herein, we review recent developments aimed at the bottom-up generation and self-assembly of starch-like materials, in order to better understand which enzymes are required for starch granule biogenesis and metabolism.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::550bd379e0b91df52f8ed5f3843d6e91Test
https://doi.org/10.3389/fbioe.2015.00136Test