التفاصيل البيبلوغرافية
العنوان: |
IL27 gene expression distinguishes multisystem inflammatory syndrome in children from febrile illness in a South African cohort |
المؤلفون: |
Spracklen, Timothy F., Mendelsohn, Simon C., Butters, Claire, Facey-Thomas, Heidi, Stander, Raphaella, Abrahams, Debbie, Erasmus, Mzwandile, Baguma, Richard, Day, Jonathan, Scott, Christiaan, Zühlke, Liesl J., Kassiotis, George, Scriba, Thomas J., Webb, Kate |
المساهمون: |
Global Challenges Research Fund, Cancer Research UK, Medical Research Council, Wellcome Trust |
المصدر: |
Frontiers in Immunology ; volume 13 ; ISSN 1664-3224 |
بيانات النشر: |
Frontiers Media SA |
سنة النشر: |
2022 |
المجموعة: |
Frontiers (Publisher - via CrossRef) |
الوصف: |
Introduction Multisystem inflammatory syndrome in children (MIS-C) is a severe acute inflammatory reaction to SARS-CoV-2 infection in children. There is a lack of data describing differential expression of immune genes in MIS-C compared to healthy children or those with other inflammatory conditions and how expression changes over time. In this study, we investigated expression of immune-related genes in South African MIS-C patients and controls. Methods The cohort included 30 pre-treatment MIS-C cases and 54 healthy non-inflammatory paediatric controls. Other controls included 34 patients with juvenile systemic lupus erythematosus, Kawasaki disease or other inflammatory conditions. Longitudinal post-treatment MIS-C specimens were available at various timepoints. Expression of 80 immune-related genes was determined by real-time quantitative PCR. Results A total of 29 differentially expressed genes were identified in pre-treatment MIS-C compared to healthy controls. Up-regulated genes were found to be overrepresented in innate immune pathways including interleukin-1 processing and pyroptosis. Post-treatment follow-up data were available for up to 1,200 hours after first treatment. All down-regulated genes and 17/18 up-regulated genes resolved to normal levels in the timeframe, and all patients clinically recovered. When comparing MIS-C to other febrile conditions, only IL27 expression could differentiate these two groups with high sensitivity and specificity. Conclusions These data indicate a unique 29-gene signature of MIS-C in South African children. The up-regulation of interleukin-1 and pyroptosis pathway genes highlights the role of the innate immune system in MIS-C. IL-27 is a potent anti-inflammatory and antiviral cytokine that may distinguish MIS-C from other conditions in our setting. |
نوع الوثيقة: |
article in journal/newspaper |
اللغة: |
unknown |
DOI: |
10.3389/fimmu.2022.992022 |
DOI: |
10.3389/fimmu.2022.992022/full |
الإتاحة: |
https://doi.org/10.3389/fimmu.2022.992022Test |
حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
رقم الانضمام: |
edsbas.C8663ADC |
قاعدة البيانات: |
BASE |