Plasma EVs Display Antigen-Presenting Characteristics in Patients With Allergic Rhinitis and Promote Differentiation of Th2 Cells
العنوان: | Plasma EVs Display Antigen-Presenting Characteristics in Patients With Allergic Rhinitis and Promote Differentiation of Th2 Cells |
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المؤلفون: | Zhi-Rou Zhou, Bi-Xin He, Xiao-Qing Liu, Ya-Qi Peng, Qing-Ling Fu, Shu-Bin Fang, De-Hua Chen, Dong Chen |
المصدر: | Frontiers in Immunology, Vol 12 (2021) Frontiers in Immunology |
بيانات النشر: | Frontiers Media SA, 2021. |
سنة النشر: | 2021 |
مصطلحات موضوعية: | Adult, CD4-Positive T-Lymphocytes, Male, Der p 1, T cell, Immunology, Antigen presentation, Severity of Illness Index, Arthropod Proteins, Flow cytometry, type 2 T helper cells, Pathogenesis, chemistry.chemical_compound, Th2 Cells, Western blot, Antigen, medicine, Humans, Immunology and Allergy, Antigens, Dermatophagoides, Original Research, allergic rhinitis, medicine.diagnostic_test, Chemistry, Vesicle, Cell Differentiation, Carboxyfluorescein succinimidyl ester, RC581-607, Rhinitis, Allergic, Molecular biology, Cysteine Endopeptidases, antigen presentation, medicine.anatomical_structure, Female, Immunologic diseases. Allergy, extracellular vesicles |
الوصف: | BackgroundAllergic rhinitis (AR) is characterized by IgE-mediated mucosa response after exposure to allergens. Extracellular vesicles (EVs) are nano-size vesicles containing biological cargos for intercellular communications. However, the role of plasma EVs in pathogenesis of AR remains largely unknown.MethodsPlasma EVs from patients with AR were isolated, quantified, and characterized. The expression of Der p 1 and antigen-presenting molecules on EVs was determined by Western blot, flow cytometry, or ELISA. PKH26- and CFSE (carboxyfluorescein succinimidyl ester)-stained AR-EVs were used to determine the uptake of EVs by CD4+T cells and their effects on CD4+T cell proliferation, respectively.ResultsPlasma EVs in healthy control (HC) and AR patients were similar in the concentration of particles, expression for specific EV markers, and both had structural lipid bilayer. However, the levels of Der p 1 on plasma EVs from both mild and moderate-severe AR patients were significantly higher than that on HC. The levels of antigen-presenting molecules on plasma EVs were similar from three subjects. Moreover, levels of Der p 1 on EVs in plasma, but not nasal secretion, were significantly associated with the symptom score of AR patients and level of plasma IL-13. Additionally, plasma EVs from patients with AR promoted the development of Th2 cells, while no effect was found on CD4+ T-cell proliferation.ConclusionsPlasma EVs derived from patients with AR exhibited antigen-presenting characteristics and promoted differentiation of Th2 cells, thus providing novel understanding of the pathogenesis of AR. |
تدمد: | 1664-3224 |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e84d8ccafd1f63dab5181b14d3b542efTest https://doi.org/10.3389/fimmu.2021.710372Test |
حقوق: | OPEN |
رقم الانضمام: | edsair.doi.dedup.....e84d8ccafd1f63dab5181b14d3b542ef |
قاعدة البيانات: | OpenAIRE |
تدمد: | 16643224 |
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