Effects of Systemic Metabolic Fuels on Glucose and Lactate Levels in the Brain Extracellular Compartment of the Mouse
| العنوان: | Effects of Systemic Metabolic Fuels on Glucose and Lactate Levels in the Brain Extracellular Compartment of the Mouse |
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| المؤلفون: | Claude Messier, Justine Courtemanche, Alexandria Béland-Millar, Tina Yuan, Jeremy Larcher |
| المصدر: | Frontiers in Neuroscience |
| بيانات النشر: | Frontiers Media S.A., 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.medical_specialty, insulin, beta-hydroxybutyrate, medicine.medical_treatment, galactose, pyruvate, Biology, Blood–brain barrier, fructose, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Internal medicine, Extracellular, medicine, Original Research, General Neuroscience, Insulin, astrocytes, Fructose, Metabolism, blood-brain barrier, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, chemistry, brain metabolism, Galactose, Fructolysis, Lactate shuttle hypothesis, 030217 neurology & neurosurgery, Neuroscience |
| الوصف: | Classic neuroenergetic research has emphasized the role of glucose, its transport and its metabolism in sustaining normal neural function leading to the textbook statement that it is the necessary and sole metabolic fuel of the mammalian brain. New evidence, including the Astrocyte-to-Neuron Lactate Shuttle hypothesis, suggests that the brain can use other metabolic substrates. To further study that possibility, we examined the effect of intraperitoneally administered metabolic fuels (glucose, fructose, lactate, pyruvate, s-hydroxybutyrate and galactose), and insulin, on blood and extracellular brain levels of glucose and lactate in the adult male CD1 mouse. Primary motor cortex extracellular levels of glucose and lactate were monitored in freely moving mice with the use of electrochemical electrodes. Blood concentration of these same metabolites were obtained by tail vein sampling and measured with glucose and lactate meters. Blood and extracellular fluctuations of glucose and lactate were monitored for a two-hour period. We found that the systemic injections of glucose, fructose, lactate, pyruvate and s-hydroxybutyrate increased blood lactate levels. Apart for a small transitory rise in brain extracellular lactate levels, the main effect of the systemic injection of glucose, fructose, lactate, pyruvate and s-hydroxybutyrate was an increase in brain extracellular glucose levels. Systemic galactose injections produced a small rise in blood glucose and lactate but almost no change in brain extracellular lactate and glucose. Systemic insulin injections led to a decrease in blood glucose and a small rise in blood lactate; however brain extracellular glucose and lactate monotonically decreased at the same rate. Our results support the concept that the brain is able to use alternative fuels and the current experiments suggest some of the mechanisms involved. |
| اللغة: | English |
| تدمد: | 1662-453X 1662-4548 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6349f3b880d856ef44014e5c75ab2a1cTest http://europepmc.org/articles/PMC5243849Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6349f3b880d856ef44014e5c75ab2a1c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1662453X 16624548 |
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