دورية أكاديمية
Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)
| العنوان: | Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) |
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| المؤلفون: | Vera Manageiro, Raquel Romão, Inês Barata Moura, Daniel A. Sampaio, Luís Vieira, Eugénia Ferreira, the Network EuSCAPE-Portugal, Manuela Caniça |
| المصدر: | Frontiers in Microbiology, Vol 9 (2018) |
| بيانات النشر: | Frontiers Media S.A. |
| سنة النشر: | 2018 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | carbapenemase-producing Enterobacteriaceae, KPC-21, EuSCAPE, Portugal, Klebsiella pneumoniae, Escherichia coli, Microbiology, QR1-502 |
| الوصف: | In Portugal, the epidemiological stage for the spread of carbapenemase-producing Enterobacteriaceae (CPE) increased from sporadic isolates or single hospital clones (2010–2013), to hospital outbreaks, later. Here we report data from a 6-month study performed under the European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE). During the study period, 67 isolates (61 Klebsiella pneumoniae and 6 Escherichia coli) non-susceptible to carbapenems were identified in participant hospital laboratories. We detected 37 blaKPC–type (including one new variant: blaKPC–21), 1 blaGES–5, and 1 blaGES–6 plus blaKPC–3, alone or in combination with other bla genes. Bioinformatics analysis of the KPC-21-producing E. coli identified the new variant blaKPC–21 in a 12,748 bp length plasmid. The blaKPC–21 gene was harbored on a non-Tn4401 element, presenting upstream a partial ISKpn6 (ΔISKpn6/ΔtraN) with the related left IR (IRL) and downstream a truncated Tn3 transposon. PFGE and MLST analysis showed an important diversity, as isolates belonged to distinct PFGE and STs profiles. In this study, we highlighted the presence of the high-risk clone E. coli sequence-type (ST) 131 clade C/H30. This worldwide disseminated E. coli lineage was already detected in Portugal among other antibiotic resistance reservoirs. This study highlights the intra- and inter-hospital spread and possible intercontinental circulation of CPE isolates. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1664-302X |
| العلاقة: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02834/fullTest; https://doaj.org/toc/1664-302XTest; https://doaj.org/article/d8564b3ee776485483734d8aa580121dTest |
| DOI: | 10.3389/fmicb.2018.02834 |
| الإتاحة: | https://doi.org/10.3389/fmicb.2018.02834Test https://doaj.org/article/d8564b3ee776485483734d8aa580121dTest |
| رقم الانضمام: | edsbas.163A5A35 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1664302X |
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| DOI: | 10.3389/fmicb.2018.02834 |