Autoreactive Peripheral Blood T Helper Cell Responses in Bullous Pemphigoid and Elderly Patients With Pruritic Disorders
| العنوان: | Autoreactive Peripheral Blood T Helper Cell Responses in Bullous Pemphigoid and Elderly Patients With Pruritic Disorders |
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| المؤلفون: | Stefan Mühlenbein, Thomas Schmidt, Michael Hertl, Farzan Solimani, Rüdiger Eming, Robert Pollmann, Milad Fehresti, Lily Holiangu, Vera Korff, Dario Didona, Luca Scarsella, Josquin Pieper, Hazem A. Juratli, Cassian Sitaru, Manuel Göbel |
| المصدر: | Frontiers in Immunology, Vol 12 (2021) Frontiers in Immunology |
| بيانات النشر: | Frontiers Media S.A., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, lcsh:Immunologic diseases. Allergy, Pemphigoid, Enzyme-Linked Immunospot Assay, pemphigoid, Dystonin, T cell, Immunology, BP180, T cells, medicine.disease_cause, Peripheral blood mononuclear cell, Autoantigens, Autoimmunity, Cohort Studies, Ointments, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Pemphigoid, Bullous, medicine, Immunology and Allergy, Humans, Glucocorticoids, Original Research, Aged, Autoantibodies, Clobetasol, integumentary system, business.industry, ELISPOT, autoimmunity, Autoantibody, T helper cell, T-Lymphocytes, Helper-Inducer, Non-Fibrillar Collagens, pruritus, medicine.disease, 030104 developmental biology, medicine.anatomical_structure, ELISpot assay, Immunoglobulin G, Cytokines, Th17 Cells, Bullous pemphigoid, business, lcsh:RC581-607 |
| الوصف: | Bullous pemphigoid (BP) is a prototypic autoimmune disorder of the elderly, characterized by serum IgG autoantibodies, namely anti-BP180 and anti-BP230, directed against components of the basal membrane zone that lead to sub-epidermal loss of adhesion. Pruritus may be indicative of a pre-clinical stage of BP, since a subset of these patients shows serum IgG autoantibodies against BP230 and/or BP180 while chronic pruritus is increasingly common in the elderly population and is associated with a variety of dermatoses. Clinical and experimental evidence further suggests that pruritus of the elderly may be linked to autoimmunity with loss of self-tolerance against cutaneous autoantigens. Thus, the objective of this study was to determine autoreactive T cell responses against BP180 in elderly patients in comparison to patients with BP. A total of 22 elderly patients with pruritic disorders, 34 patients with bullous or non-bullous BP and 34 age-matched healthy controls were included in this study. The level of anti-BP180 and anti-BP230 IgG serum autoantibodies, Bullous Pemphigoid Disease Area Index (BPDAI), and pruritus severity were assessed for all patients and controls. For characterization of the autoreactive T cell response, peripheral blood mononuclear cells were stimulated ex vivo with recombinant BP180 proteins (NH2- and COOH-terminal domains) and the frequencies of BP180-specific T cells producing interferon-γ, interleukin (IL)-5 or IL-17 were subsequently determined by ELISpot assay. Patients with BP showed a mixed Th1/Th2 response against BP180 while autoreactive Th1 cells were identified in a minor subset of elderly patients with pruritic disorders. Furthermore, our T cell characterization revealed that therapeutic application of topical clobetasol propionate ointment in BP patients significantly reduced peripheral blood BP180-specific T cells, along with clinically improved symptoms, strongly suggesting a systemic immunosuppressive effect of this treatment. |
| اللغة: | English |
| تدمد: | 1664-3224 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::633503a1965bf558d2473cbd9b5c0e11Test https://www.frontiersin.org/articles/10.3389/fimmu.2021.569287/fullTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....633503a1965bf558d2473cbd9b5c0e11 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16643224 |
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