دورية أكاديمية
Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro
| العنوان: | Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro |
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| المؤلفون: | Keqin Li, Shuai Shao, Tongjie Ji, Min Liu, Lufeng Wang, Ying Pang, Mu Chen, Siyi Xu, Kuiming Zhang, Qi Wang, Zhongwei Zhuang, Liang Wei, Yanfei Zhang, Yanlin Chen, Yang Wang, Jing Zhang, Kui Chen, Hao Lian, Chunlong Zhong |
| المصدر: | Frontiers in Neuroanatomy, Vol 15 (2021) |
| بيانات النشر: | Frontiers Media S.A., 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Neurosciences. Biological psychiatry. Neuropsychiatry LCC:Human anatomy |
| مصطلحات موضوعية: | capicua, transcriptional repressor, dendritic growth, spine growth, Ets factors, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571, Human anatomy, QM1-695 |
| الوصف: | Capicua (Cic), a transcriptional repressor frequently mutated in brain cancer oligodendroglioma, is highly expressed in adult neurons. However, its function in the dendritic growth of neurons in the hippocampus remains poorly understood. Here, we confirmed that Cic was expressed in hippocampal neurons during the main period of dendritogenesis, suggesting that Cic has a function in dendrite growth. Loss-of-function and gain-of function assays indicated that Cic plays a central role in the inhibition of dendritic morphogenesis and dendritic spines in vitro. Further studies showed that overexpression of Cic reduced the expression of Ets in HT22 cells, while in vitro knockdown of Cic in hippocampal neurons significantly elevated the expression of Ets. These results suggest that Cic may negatively control dendrite growth through Ets, which was confirmed by ShRNA knockdown of either Etv4 or Etv5 abolishing the phenotype of Cic knockdown in cultured neurons. Taken together, our results suggest that Cic inhibits dendritic morphogenesis and the growth of dendritic spines through Ets. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1662-5129 |
| العلاقة: | https://www.frontiersin.org/articles/10.3389/fnana.2021.669310/fullTest; https://doaj.org/toc/1662-5129Test |
| DOI: | 10.3389/fnana.2021.669310 |
| الوصول الحر: | https://doaj.org/article/5918075d2328459e9ed8f90301d4fe9eTest |
| رقم الانضمام: | edsdoj.5918075d2328459e9ed8f90301d4fe9e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16625129 |
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| DOI: | 10.3389/fnana.2021.669310 |