Recent Progress in Chemo-Enzymatic Methods for the Synthesis of N-Glycans
| العنوان: | Recent Progress in Chemo-Enzymatic Methods for the Synthesis of N-Glycans |
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| المؤلفون: | Meng-Hai Xiang, Xiao-Dong Gao, Yi Ding, Ning Wang, Zheng-Hui Chen, Qiang Chao |
| المصدر: | Frontiers in Chemistry, Vol 8 (2020) Frontiers in Chemistry |
| بيانات النشر: | Frontiers Media S.A., 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Glycan, Glycosylation, 02 engineering and technology, Computational biology, Review, N-glycosylation, homogeneous glycoprotein, 010402 general chemistry, 01 natural sciences, Chemical synthesis, lcsh:Chemistry, chemistry.chemical_compound, N-linked glycosylation, Glycosyltransferase, glycosyltransferases, Asparagine, chemistry.chemical_classification, biology, Chemistry, General Chemistry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, carbohydrates (lipids), lcsh:QD1-999, glycosidase, biology.protein, biomarker, Protein folding, glycosynthase, 0210 nano-technology, Glycoprotein, chemo-enzymatic synthesis |
| الوصف: | Asparagine (N)-linked glycosylation is one of the most common co- and post-translational modifications of both intra- and extracellularly distributing proteins, which directly affects their biological functions, such as protein folding, stability and intercellular traffic. Production of the structural well-defined homogeneous N-glycans contributes to comprehensive investigation of their biological roles and molecular basis. Among the various methods, chemo-enzymatic approach serves as an alternative to chemical synthesis, providing high stereoselectivity and economic efficiency. This review summarizes some recent advances in the chemo-enzymatic methods for the production of N-glycans, including the preparation of substrates and sugar donors, and the progress in the glycosyltransferases characterization which leads to the diversity of N-glycan synthesis. We discuss the bottle-neck and new opportunities in exploiting the chemo-enzymatic synthesis of N-glycans based on our research experiences. In addition, downstream applications of the constructed N-glycans, such as automation devices and homogeneous glycoproteins synthesis are also described. |
| اللغة: | English |
| تدمد: | 2296-2646 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2246590763bf2fd80f0ec4db78bedfadTest https://www.frontiersin.org/article/10.3389/fchem.2020.00513/fullTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2246590763bf2fd80f0ec4db78bedfad |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22962646 |
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