المؤلفون: Aina Teniente-Serra, Eduarda Pizarro, Bibiana Quirant-Sánchez, Marco A. Fernández, Marta Vives-Pi, Eva M. Martinez-Caceres

المصدر: Frontiers in Immunology, Vol 12 (2021)

مصطلحات موضوعية: immunomonitoring, lymphocyte subpopulations, flow cytometry, type 1 diabetes, onset, adult, Immunologic diseases. Allergy, RC581-607

الوصف: T- and B-lymphocytes play an important role in the pathogenesis of type 1 diabetes (T1D), a chronic disease caused by the autoimmune destruction of the insulin-producing cells in the pancreatic islets. Flow cytometry allows their characterization in peripheral blood, letting to investigate changes in cellular subpopulations that can provide insights in T1D pathophysiology. With this purpose, CD4+ and CD8+ T cells (including naïve, central memory, effector memory and terminally differentiated effector (TEMRA), Th17 and Tregs) and B cells subsets (naïve, unswitched memory, switched memory and transitional B cells) were analysed in peripheral blood of adult T1D patients at disease onset and after ≥2 years using multiparametric flow cytometry. Here we report changes in the percentage of early and late effector memory CD4+ and CD8+ T cells as well as of naïve subsets, regulatory T cells and transitional B cells in peripheral blood of adult patients at onset of T1D when compared with HD. After 2 years follow-up these changes were maintained. Also, we found a decrease in percentage of Th17 and numbers of T cells with baseline. In order to identify potential biomarkers of disease, ROC curves were performed being late EM CD4 T cell subset the most promising candidate. In conclusion, the observed changes in the percentage and/or absolute number of lymphocyte subpopulations of adult T1D patients support the hypothesis that effector cells migrate to the pancreas and this autoimmune process perseveres along the disease. Moreover, multiparametric flow allows to identify those subsets with potential to be considered biomarkers of disease.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fimmu.2021.784110/fullTest; https://doaj.org/toc/1664-3224Test

الوصول الحر: https://doaj.org/article/f723e059b91945f79b9a4dc6093c1101Test

المؤلفون: Amund Holte Berger, Eirik Bratland, Thea Sjøgren, Marte Heimli, Torgeir Tyssedal, Øyvind Bruserud, Stefan Johansson, Eystein Sverre Husebye, Bergithe Eikeland Oftedal, Anette Susanne Bøe Wolff

المصدر: Frontiers in Immunology, Vol 12 (2021)
Frontiers in Immunology

مصطلحات موضوعية: Adult, Male, Immunology, autoimmune disease, autoimmune polyendocrine syndrome type 1, Biology, medicine.disease_cause, T-Lymphocytes, Regulatory, Autoimmunity, Transcriptome, transcriptomics, Downregulation and upregulation, medicine, Immunology and Allergy, Humans, APS-1, RNAseq analysis, Intestinal Mucosa, Polyendocrinopathies, Autoimmune, Original Research, Autoimmune disease, flow cytometry, Middle Aged, RC581-607, medicine.disease, Autoimmune regulator, Lipid Metabolism, Fatty Acid Synthase, Type I, Autoimmune polyendocrine syndrome type 1, Tregs (regulatory T cells), Autoimmune polyendocrine syndrome, Case-Control Studies, Female, Immunologic diseases. Allergy, Homing (hematopoietic)

الوصف: Autoimmune polyendocrine syndrome type I (APS-1) is a monogenic model disorder of organ-specific autoimmunity caused by mutations in theAutoimmune regulator (AIRE)gene. AIRE facilitates the expression of organ-specific transcripts in the thymus, which is essential for efficient removal of dangerous self-reacting T cells and for inducing regulatory T cells (Tregs). Although reduced numbers and function of Tregs have been reported in APS-I patients, the impact of AIRE deficiency on gene expression in these cells is unknown. Here, we report for the first time on global transcriptional patterns of isolated Tregs from APS-1 patients compared to healthy subjects. Overall, we found few differences between the groups, although deviant expression was observed for the genesTMEM39B, SKIDA1, TLN2, GPR15, FASN, BCAR1, HLA-DQA1, HLA-DQB1, HLA-DRA, GPSM3 and AKR1C3.Of significant interest, the consistent downregulation ofGPR15may indicate failure of Treg gut homing which could be of relevance for the gastrointestinal manifestations commonly seen in APS-1. UpregulatedFASNexpression in APS-1 Tregs points to increased metabolic activity suggesting a putative link to faulty Treg function. Functional studies are needed to determine the significance of these findings for the immunopathogenesis of APS-1 and for Treg immunobiology in general.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16bd36d109e668fc4e9367afdebd231eTest
https://www.frontiersin.org/articles/10.3389/fimmu.2021.722860/fullTest