دورية أكاديمية
Assessment of an antibody-in-lymphocyte supernatant assay for the etiological diagnosis of pneumococcal pneumonia in children
| العنوان: | Assessment of an antibody-in-lymphocyte supernatant assay for the etiological diagnosis of pneumococcal pneumonia in children |
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| المؤلفون: | Carter, MJ, Gurung, P, Jones, C, Rajkarnikar, S, Kandasamy, R, Gurung, M, Thorson, S, Gautam, MC, Prajapati, KG, Khadka, B, Maharjan, A, Knight, JC, Murdoch, DR, Darton, TC, Voysey, M, Wahl, B, O'Brien, KL, Kelly, S, Ansari, I, Shah, G, Ekström, N, Melin, M, Pollard, AJ, Kelly, DF, Shrestha, S |
| بيانات النشر: | Frontiers Media |
| سنة النشر: | 2020 |
| المجموعة: | Oxford University Research Archive (ORA) |
| الوصف: | New diagnostic tests for the etiology of childhood pneumonia are needed. We evaluated the antibody-in-lymphocyte supernatant (ALS) assay to detect immunoglobulin (Ig) G secretion from ex vivo peripheral blood mononuclear cell (PBMC) culture, as a potential diagnostic test for pneumococcal pneumonia. We enrolled 348 children with pneumonia admitted to Patan Hospital, Kathmandu, Nepal between December 2015 and September 2016. PBMCs sampled from participants were incubated for 48 h before harvesting of cell culture supernatant (ALS). We used a fluorescence-based multiplexed immunoassay to measure the concentration of IgG in ALS against five conserved pneumococcal protein antigens. Of children with pneumonia, 68 had a confirmed etiological diagnosis: 12 children had pneumococcal pneumonia (defined as blood or pleural fluid culture-confirmed; or plasma CRP concentration ≥60 mg/l and nasopharyngeal carriage of serotype 1 pneumococci), and 56 children had non-pneumococcal pneumonia. Children with non-pneumococcal pneumonia had either a bacterial pathogen isolated from blood (six children); or C-reactive protein <60 mg/l, absence of radiographic consolidation and detection of a pathogenic virus by multiplex PCR (respiratory syncytial virus, influenza viruses, or parainfluenza viruses; 23 children). Concentrations of ALS IgG to all five pneumococcal proteins were significantly higher in children with pneumococcal pneumonia than in children with non-pneumococcal pneumonia. The concentration of IgG in ALS to the best-performing antigen discriminated between children with pneumococcal and non-pneumococcal pneumonia with a sensitivity of 1.0 (95% CI 0.73–1.0), specificity of 0.66 (95% CI 0.52–0.78) and area under the receiver-operating characteristic curve (AUROCC) 0.85 (95% CI 0.75–0.94). Children with pneumococcal pneumonia were older than children with non-pneumococcal pneumonia (median 5.6 and 2.0 years, respectively, p < 0.001). When the analysis was limited to children ≥2 years of age, assay of IgG ALS to ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://ora.ox.ac.uk/objects/uuid:0a64a49a-97c9-4099-8c38-66e9fe89bd92Test; https://doi.org/10.3389/fcimb.2019.00459Test |
| DOI: | 10.3389/fcimb.2019.00459 |
| الإتاحة: | https://doi.org/10.3389/fcimb.2019.00459Test https://ora.ox.ac.uk/objects/uuid:0a64a49a-97c9-4099-8c38-66e9fe89bd92Test |
| حقوق: | info:eu-repo/semantics/openAccess ; CC Attribution (CC BY) |
| رقم الانضمام: | edsbas.3D676196 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fcimb.2019.00459 |
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