Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2
| العنوان: | Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2 |
|---|---|
| المؤلفون: | Sabina Honisch, Lutz Birnbaumer, Syed M. Qadri, Christine Zelenak, Guilai Liu, Adrian Lupescu, Birgit Fehrenbacher, Anja T. Umbach, Florian Lang, Hong Chen, Andreas F. Mack, Rexhep Rexhepaj, Guoxing Liu, Rosi Bissinger, Yogesh Singh, Elisabeth Lang, Martin Schaller, Mehrdad Ghashghaeinia, Hajar Fakhri |
| المصدر: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Scientific Reports. 2016;6(1):1-10 Repositorio Institucional (UCA) Pontificia Universidad Católica Argentina instacron:UCA Scientific Reports |
| بيانات النشر: | Freie Universität Berlin, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Erythrocyte Indices, 0301 basic medicine, G Protein, Programmed cell death, Ceramide, CIENCIAS MÉDICAS Y DE LA SALUD, Erythrocytes, genetic structures, Cell Survival, G protein, Inmunología, Eryptosis, BIOLOGIA CELULAR, Apoptosis, Phosphatidylserines, Biology, Article, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Animals, Humans, Gi2alpha, PROTEINA, Erythocyte, Mice, Knockout, Multidisciplinary, Phosphatidylserine, purl.org/becyt/ford/3.1 [https], Molecular biology, 3. Good health, Cell biology, Medicina Básica, Cytosol, 030104 developmental biology, Erythrocyte maturation, chemistry, CELULAS DE LA SANGRE, 030220 oncology & carcinogenesis, purl.org/becyt/ford/3 [https], GTP-Binding Protein alpha Subunit, Gi2, Sphingomyelin |
| الوصف: | Fil: Bissinger, Rosi. University of Tuebingen. Vascular Medicine and Physiology. Institute of Cardiology; Alemania Fil: Lang, Elisabeth. University of Duesseldorf. Hepatology and Infectious Diseases. Department of Gastroenterology; Alemania Fil: Ghashghaeinia, Mehrdad. University of Tuebingen. Vascular Medicine and Physiology. Institute of Cardiology; Alemania Fil: Zelenak, Christine. Charité Medical University. Department of Internal Medicine; Alemania Fil: Fehrenbacher, Birgit. University of Tuebingen. Department of Dermatology; Alemania Fil: Honisch, Sabina. University of Tuebingen. Vascular Medicine and Physiology. Institute of Cardiology; Alemania Fil: Chen, Hong. University of Tuebingen. Vascular Medicine and Physiology. Institute of Cardiology; Alemania Fil: Fakhri, Hajar. University of Tuebingen. Vascular Medicine and Physiology. Institute of Cardiology; Alemania Fil: Umbach, Anja T. University of Tuebingen. Vascular Medicine and Physiology. Institute of Cardiology; Alemania Fil: Liu, Guilai. University of Tuebingen. Vascular Medicine and Physiology. Institute of Cardiology; Alemania Fil: Rexhepaj, Rexhep. University of Tuebingen. Vascular Medicine and Physiology. Institute of Cardiology; Alemania Fil: Rexhepaj, Rexhep. University of Bonn. Institute of Biochemistry and Molecular Biology; Alemania Fil: Liu, Guoxing. University of Tuebingen. Vascular Medicine and Physiology. Institute of Cardiology; Alemania Fil: Schaller, Martin. University of Tuebingen. Department of Dermatology; Alemania Fil: Mack, Andreas F. University of Tuebingen. Institute of Anatomy; Alemania Fil: Lupescu, Adrian. University of Tuebingen. Vascular Medicine and Physiology. Institute of Cardiology; Alemania Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina Fil: Birnbaumer, Lutz. National Institute of Health. Neurobiology Laboratory; Estados Unidos Fil: Lang, Florian. University of Tuebingen. Vascular Medicine and Physiology. Institute of Cardiology; Alemania Fil: Qadri, Syed M. University of Tuebingen. Vascular Medicine and Physiology. Institute of Cardiology; Alemania Fil: Qadri, Syed M. McMaster University. Department of Pathology and Molecular Medicine; Canadá Abstract: Putative functions of the heterotrimeric G-protein subunit Gαi2-dependent signaling include ion channel regulation, cell differentiation, proliferation and apoptosis. Erythrocytes may, similar to apoptosis of nucleated cells, undergo eryptosis, characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine (PS) exposure. Eryptosis may be triggered by increased cytosolic Ca(2+) activity and ceramide. In the present study, we show that Gαi2 is expressed in both murine and human erythrocytes and further examined the survival of erythrocytes drawn from Gαi2-deficient mice (Gαi2(-/-)) and corresponding wild-type mice (Gαi2(+/+)). Our data show that plasma erythropoietin levels, erythrocyte maturation markers, erythrocyte counts, hematocrit and hemoglobin concentration were similar in Gαi2(-/-) and Gαi2(+/+) mice but the mean corpuscular volume was significantly larger in Gαi2(-/-) mice. Spontaneous PS exposure of circulating Gαi2(-/-) erythrocytes was significantly lower than that of circulating Gαi2(+/+) erythrocytes. PS exposure was significantly lower in Gαi2(-/-) than in Gαi2(+/+) erythrocytes following ex vivo exposure to hyperosmotic shock, bacterial sphingomyelinase or C6 ceramide. Erythrocyte Gαi2 deficiency further attenuated hyperosmotic shock-induced increase of cytosolic Ca(2+) activity and cell shrinkage. Moreover, Gαi2(-/-) erythrocytes were more resistant to osmosensitive hemolysis as compared to Gαi2(+/+) erythrocytes. In conclusion, Gαi2 deficiency in erythrocytes confers partial protection against suicidal cell death. |
| وصف الملف: | application/pdf |
| اللغة: | Hungarian |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad2d9ce34b64c8ae33ee6231a461e835Test http://www.nature.com/articles/srep30925Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ad2d9ce34b64c8ae33ee6231a461e835 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |