Ring Finger Protein 11 Inhibits Melanocortin 3 and 4 Receptor Signaling
| العنوان: | Ring Finger Protein 11 Inhibits Melanocortin 3 and 4 Receptor Signaling |
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| المؤلفون: | Anne Müller, Lars Niederstadt, Wenke Jonas, Chun-Xia Yi, Franziska Meyer, Petra Wiedmer, Jana Fischer, Carsten Grötzinger, Annette Schürmann, Matthias Tschöp, Gunnar Kleinau, Annette Grüters, Heiko Krude, Heike Biebermann |
| المساهمون: | AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Endocrinology |
| المصدر: | Frontiers in endocrinology, 7:109 Front. Endocrin. 7:109 (2016) Frontiers in Endocrinology, Vol 7 (2016) Frontiers in endocrinology, 7. Frontiers Media S.A. Frontiers in Endocrinology |
| بيانات النشر: | Freie Universität Berlin, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Receptor expression, Inflammation, G protein coupled receptor, Biology, 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit, lcsh:Diseases of the endocrine glands. Clinical endocrinology, 03 medical and health sciences, Endocrinology, 0302 clinical medicine, Internal medicine, medicine, Receptor, Original Research, G protein-coupled receptor, protein network, lcsh:RC648-665, Melanocortin 3 receptor, Melanocortin 4 receptor, 030104 developmental biology, weight regulation, inflammation, G Protein Coupled Receptor, Protein Complementation Assay, Protein Network, Weight Regulation, Tumor necrosis factor alpha, medicine.symptom, Melanocortin, protein complementation assay, 030217 neurology & neurosurgery, hormones, hormone substitutes, and hormone antagonists |
| الوصف: | Intact melanocortin signaling via the G protein-coupled receptors (GPCRs), melanocortin receptor 4 (MC4R), and melanocortin receptor 3 (MC3R) is crucial for body weight maintenance. So far, no connection between melanocortin signaling and hypothalamic inflammation has been reported. Using a bimolecular fluorescence complementation library screen, we identified a new interaction partner for these receptors, ring finger protein 11 (RNF11). RNF11 participates in the constitution of the A20 complex that is involved in reduction of tumor necrosis factor α (TNFα)-induced NFκB signaling, an important pathway in hypothalamic inflammation. Mice treated with high-fat diet (HFD) for 3 days demonstrated a trend toward an increase in hypothalamic Rnf11 expression, as shown for other inflammatory markers under HFD. Furthermore, Gs-mediated signaling of MC3/4R was demonstrated to be strongly reduced to 20-40% by co-expression of RNF11 despite unchanged total receptor expression. Cell surface expression was not affected for MC3R but resulted in a significant reduction of MC4R to 61% by co-expression with RNF11. Mechanisms linking HFD, inflammation, and metabolism remain partially understood. In this study, a new axis between signaling of specific body weight regulating GPCRs and factors involved in hypothalamic inflammation is suggested. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1664-2392 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2047967e5c6904a2dd0c25d8a198bfbeTest https://doi.org/10.17169/refubium-25040Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2047967e5c6904a2dd0c25d8a198bfbe |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16642392 |
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