دورية أكاديمية
Combining DOE with neurofuzzy logic for healthy mineral nutrition of pistachio rootstocks in vitro culture
| العنوان: | Combining DOE with neurofuzzy logic for healthy mineral nutrition of pistachio rootstocks in vitro culture |
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| المؤلفون: | Nezami Alanagh, Esmaeil, Garoosi, Ghasem-Ali, Landín Pérez, Mariana, Gallego, Pedro Pablo |
| المساهمون: | Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica |
| بيانات النشر: | Fontiers Media |
| المجموعة: | Minerva - Repositorio institucional da Universidade de Santiago de Compostela (USC) |
| مصطلحات موضوعية: | DOE, Micropropagation, Health shoots, Neurofuzzy logic, Pistachio rootstock, Physiological disorders |
| الوصف: | The aim of this study was to determine the effects of Murashige and Skoog (MS) salts on optimal growth of two pistachio rootstocks, P. vera cv. “Ghazvini” and “UCB1” using design of experiments (DOE) and artificial intelligence (AI) tools. MS medium with 14 macro—and micro-elements was used as base point and its concentration varied from 0 to 5 × MS concentrations. Design of experiments (DOE) software was used to generate a five-dimensional design space by categorizing MS salts into five independent factors (NH4NO3, KNO3, mesos, micros and iron), reducing the experimental design space from 3,125 to just 29 treatments. Typical plant growth parameters such as shoot quality (SQ), proliferation rate (PR), shoot length (SL), and some physiological disorders including shoot-tip necrosis (STN) and callus formation at the base of explants (BC) were evaluated for each treatment. The results were successfully modeled using neurofuzzy logic software. The model delivered new insights, by different sets of “IF–THEN” rules, pinpointing the key role of some ion interactions (SO42- × Cl−, K+ × SO42- × EDTA−, and Fe2+ × Cu2+ × NO3-) for SQ, PR, and SL, whilst physiological disorders (STN and BC) were governed mainly by independent ions as Fe2+ and EDTA−, respectively. In our opinion, the methodology and results obtained in this study is extremely useful to understand the effect of mineral nutrients on pistachio in vitro culture, through discovering new complex interactions among macro—and micro-elements which can be implemented to design new media of plant tissue culture and improve healthy plant micropropagation for any plant species ; An especial thanks also goes to Xunta de Galicia of Spain, for financial support (Competitive Reference Groups, ED431C2017/09-FEDER and Programa REDES ED431D-2017/18, respectively) ; SI |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1664-462X |
| العلاقة: | https://doi.org/10.3389/fpls.2018.01474Test; Nezami-Alanagh E, Garoosi G-A, Landín M and Gallego PP (2018) Combining DOE With Neurofuzzy Logic for Healthy Mineral Nutrition of Pistachio Rootstocks in vitro Culture. Front. Plant Sci. 9:1474. doi:10.3389/fpls.2018.01474; http://hdl.handle.net/10347/22504Test |
| DOI: | 10.3389/fpls.2018.01474 |
| الإتاحة: | https://doi.org/10.3389/fpls.2018.01474Test http://hdl.handle.net/10347/22504Test |
| حقوق: | Copyright © 2018 Nezami-Alanagh, Garoosi, Landín and Gallego. This is an openaccess article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms ; https://creativecommons.org/licenses/by/4.0Test/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.81886C1D |
| قاعدة البيانات: | BASE |
| تدمد: | 1664462X |
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| DOI: | 10.3389/fpls.2018.01474 |