Preferential expansion of human umbilical cord blood-derived CD34-positive cells on major histocompatibility complex-matched amnion-derived mesenchymal stem cells
| العنوان: | Preferential expansion of human umbilical cord blood-derived CD34-positive cells on major histocompatibility complex-matched amnion-derived mesenchymal stem cells |
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| المؤلفون: | Naoki Hosaka, Satoshi Okazaki, Junko Kato, Hiroko Hisha, Xiaoli Wang, Changye Song, Qing Li, Hideharu Kanzaki, Takashi Takaki, Muneo Inaba, Susumu Ikehara, Tomomi Mizokami |
| المصدر: | Haematologica. 94:618-628 |
| بيانات النشر: | Ferrata Storti Foundation (Haematologica), 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, CD34, Antigens, CD34, HLA-C Antigens, Biology, Major Histocompatibility Complex, Chondrocytes, Microscopy, Electron, Transmission, Pregnancy, Adipocytes, medicine, Humans, Cell Lineage, Amnion, Cells, Cultured, Cell Proliferation, Stem cell transplantation for articular cartilage repair, Osteoblasts, HLA-A Antigens, Cell Differentiation, Mesenchymal Stem Cells, Amniotic stem cells, Hematology, Fetal Blood, Flow Cytometry, Coculture Techniques, Cord lining, Cell biology, Endothelial stem cell, HLA-B Antigens, Amniotic epithelial cells, Leukocytes, Mononuclear, Cytokines, Original Article, Female, Stem cell, Adult stem cell |
| الوصف: | Background We previously found in a murine hematopoietic system that hematopoietic stem cells show high differentiation and proliferation capacity on bone marrow-derived mesenchymal stem cells/stromal cells (microenvironment) with “self” major histocompatibility complex (MHC).Design and Methods We examined whether amnion-derived adherent cells have the characteristics of mesenchymal stem cells, and whether these adherent cells can support the proliferation of umbilical cord blood-derived lineage-negative and CD34-positive cells (Lin–CD34+ cells) obtained from the same fetus to a greater extent than those derived from other fetuses.Results Culture-expanded amnion-derived adherent cells expressed mesenchymal stem cell markers and HLA-ABC molecules and could differentiate into osteoblasts, adipocytes and chondrocyte-like cells, indicating that the cells have the characteristics of mesenchymal stem cells. The Lin–CD34+ cells purified from the frozen umbilical cord blood were strongly positive for HLA-ABC, and contained a large number of hematopoietic stem cells. When the Lin–CD34+ cells were cultured on the autologous (MHC-matched) or MHC-mismatched amnion-derived adherent cells in short-term assays (hematopoietic stem cell-proliferation) and long-term culture-initiating cell assays, greater expansion of the Lin–CD34+ cells was observed in the MHC-matched combination than in MHC-mismatched combinations. The concentration of granulocyte-macrophage colony-stimulating factor in the culture supernatants of the long-term culture-initiating cell assays was significantly higher in the MHC-matched combination than in MHC-mismatched combinations.Conclusions It is likely that a MHC restriction exists between hematopoietic stem cells and mesenchymal stem cells/stromal cells in the human hematopoietic system and that granulocute-macropage colony-stimulating factor contributes to some extent to the preferential hematopoiesis-supporting ability of the MHC-matched amnion-derived adherent cells. |
| تدمد: | 1592-8721 0390-6078 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3d69ca3ed5a9c84acc703c3fb61753dTest https://doi.org/10.3324/haematol.2008.004705Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c3d69ca3ed5a9c84acc703c3fb61753d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15928721 03906078 |
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