دورية أكاديمية
Modulating effect of serum on the stimulation of plasminogen activator inhibitor 2 production in human gingival fibroblasts by lipopolysaccharide and interleukin-1beta
| العنوان: | Modulating effect of serum on the stimulation of plasminogen activator inhibitor 2 production in human gingival fibroblasts by lipopolysaccharide and interleukin-1beta |
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| المؤلفون: | Xiao, Y., Bartold, P. |
| بيانات النشر: | FDI World Dental Press Ltd. |
| سنة النشر: | 2004 |
| المجموعة: | The University of Adelaide: Digital Library |
| مصطلحات موضوعية: | Cells, Cultured, Fibroblasts, Fetal Blood, Gingiva, Animals, Cattle, Humans, Lipopolysaccharides, Plasminogen Activator Inhibitor 2, Interleukin-1, Culture Media, Conditioned, Blotting, Western, Northern, Analysis of Variance, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Regulation, Up-Regulation, Dose-Response Relationship, Drug |
| الوصف: | Copyright © 2004 Journal of the International Academy of Periodontology ; Objective Plasminogen activator inhibitor-2 (PAI-2) is an important counter proteolysis factor which helps protect tissues from inflammatory stress. The expression of PAI-2 can be modulated by various inflammatory stimulants and mediators. The aim of the present study was to investigate how serum factors, might modulate the effects of lipopolysaccharide (LPS) and interleukin-1beta on PAI-2 production by human gingival fibroblasts. Methods Human gingival fibroblasts were exposed to LPS derived from Actinobacillus actinomycetemcomitans or Escherichia coli and a commercial source of interleukin-1beta (IL-1beta). The expression of PAI-2 and its mRNA was monitored by Western blotting, RT-PCR, and Northern blotting. Results The results showed that the distribution of PAI-2 synthesised by human gingival fibroblasts (HGF) was mostly as an intracellular protein (47kDa). The presence of serum in the culture media was absolutely necessary for both the secretion of PAI-2 and for the effect of the inflammatory mediators (LPS and IL-1beta). A pattern of PAI-2 response in HGF after LPS stimulation was detected with a quick up-regulation of the PAI-2 mRNA, which was down regulated when extracellular PAI-2 (60kDa mass) levels reached plateau levels. The synthesis of PAI-2 by HGF, in terms of mRNA expression and protein synthesis, was more sensitive to stimulation with lower concentrations of LPS (10 ng/ml) than with higher LPS concentrations. Conclusions PAI-2 is a serum dependent molecule with major cytosolic localisation in HGF. Its cellular accumulation and secretion can be modulated by bacterial LPS and IL-1beta through serum factors. ; Y. Xiao and P.M. Bartold |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1466-2094 2518-3745 |
| العلاقة: | Journal of the International Academy of Periodontology, 2004; 6(3):81-88; http://hdl.handle.net/2440/39034Test; Bartold, P. [0000-0002-5695-3877] |
| الإتاحة: | http://hdl.handle.net/2440/39034Test |
| رقم الانضمام: | edsbas.B0107033 |
| قاعدة البيانات: | BASE |
| تدمد: | 14662094 25183745 |
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