Effect of gardenia tea on apoptosis of human thyroid cancer cell line SW579 through PI3K-Akt pathway
| العنوان: | Effect of gardenia tea on apoptosis of human thyroid cancer cell line SW579 through PI3K-Akt pathway |
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| المؤلفون: | Peng MA, Yi WU, Lin TAO, Jianli HAN |
| المصدر: | Food Science and Technology v.42 2022 Food Science and Technology (Campinas) Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
| بيانات النشر: | FapUNIFESP (SciELO), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | functional food, PI3K-Akt pathway, human thyroid cancer cell line SW579, anticancer, gardenia tea, Food Science, Biotechnology |
| الوصف: | The purpose of this study was to observe its in vitro inhibitory effect on human thyroid cancer cell line SW579 through PI3K-Akt pathway. In this study, the survival of cells and the inhibitory effect of gardenia tea extract (GTE) on SW579 cancer cells were firstly observed by MTT method. Further qPCR experiments were used to observe mRNA expression in SW579 cancer cells. The active compound composition in the GTE was then also determined by HPLC. The experimental results showed that GTE had almost no effect on normal primary thyroid cells and had no toxic effect, but GTE could significantly inhibit the proliferation of SW579 cancer cells. The detection of mRNA expression in SW579 cancer cells showed that GTE could up-regulate the expressions of Bax, caspase-3, caspase-8 and caspase-9 and down-regulate the expressions of Bcl-2, Bcl-xL, PI3K and Akt in SW579 cancer cells. The chemical composition test showed that GTE mainly contains three compounds, namely rutin, quercetin and phloretin. It can be seen that gardenia tea can regulate the PI3K-Akt pathway of SW579 cancer cells through the effective active substances contained in it, and exert its anti-cancer effect. It is a health-care tea with good anti-cancer effect. |
| وصف الملف: | text/html |
| تدمد: | 1678-457X 0101-2061 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0335d8f4d5b9e2f5e4451396d074790eTest https://doi.org/10.1590/fst.73122Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0335d8f4d5b9e2f5e4451396d074790e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1678457X 01012061 |
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