SIRT1 negatively regulates amyloid-beta-induced inflammation via the NF-κB pathway
| العنوان: | SIRT1 negatively regulates amyloid-beta-induced inflammation via the NF-κB pathway |
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| المؤلفون: | Lining Cao, Fengyun Wang, Chaoqi Liu, Honggeng Wang |
| المصدر: | Brazilian Journal of Medical and Biological Research Brazilian Journal of Medical and Biological Research, Vol 46, Iss 8, Pp 659-669 (2013) Brazilian Journal of Medical and Biological Research v.46 n.8 2013 Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Volume: 46, Issue: 8, Pages: 659-669, Published: AUG 2013 |
| بيانات النشر: | FapUNIFESP (SciELO), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Retinal degeneration, Physiology, Retinal Pigment Epithelium, SRT1720, Biochemistry, Antioxidants, Macular Degeneration, chemistry.chemical_compound, Sirtuin 1, Blood-Retinal Barrier, Stilbenes, General Pharmacology, Toxicology and Pharmaceutics, lcsh:QH301-705.5, lcsh:R5-920, biology, General Neuroscience, NF-kappa B, Interleukin, Matrix metalloproteinase-9, General Medicine, Cell biology, Matrix Metalloproteinase 9, RNA Interference, Amyloid-beta, medicine.symptom, lcsh:Medicine (General), Signal Transduction, Adult, Cell Survival, Amyloid beta, Primary Cell Culture, Immunology, Biophysics, Inflammation, Real-Time Polymerase Chain Reaction, Heterocyclic Compounds, 4 or More Rings, medicine, Humans, Gene Silencing, Tight junction, Enzyme Assays, Amyloid beta-Peptides, Interleukin-6, Age-related macular degeneration, Interleukin-8, Biomedical Sciences, Barrier integrity, NF-κB, Cell Biology, medicine.disease, IκBα, lcsh:Biology (General), chemistry, Resveratrol, biology.protein, sense organs |
| الوصف: | Chronic inflammation induced by amyloid-beta (Aβ) plays a key role in the development of age-related macular degeneration (AMD), and matrix metalloproteinase-9 (MMP-9), interleukin (IL)-6, and IL-8 may be associated with chronic inflammation in AMD. Sirtuin 1 (SIRT1) regulates inflammation via inhibition of nuclear factor-kappa B (NF-κB) signaling, and resveratrol has been reported to prevent Aβ-induced retinal degeneration; therefore, we investigated whether this action was mediated via activation of SIRT1 signaling. Human adult retinal pigment epithelial (RPE) cells were exposed to Aβ, and overactivation and knockdown of SIRT1 were performed to investigate whether SIRT1 is required for abrogating Aβ-induced inflammation. We found that Aβ-induced RPE barrier disruption and expression of IL-6, IL-8, and MMP-9 were abrogated by the SIRT1 activator SRT1720, whereas alterations induced by Aβ in SIRT1-silenced RPE cells were not attenuated by SRT1720. In addition, SRT1720 inhibited Aβ-mediated NF-κB activation and decrease of the NF-κB inhibitor, IκBα. Our findings suggest a protective role for SIRT1 signaling in Aβ-dependent retinal degeneration and inflammation in AMD. |
| وصف الملف: | text/html |
| تدمد: | 0100-879X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8330d8d710754ca1fddaded0476f7eceTest https://doi.org/10.1590/1414-431x20132903Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8330d8d710754ca1fddaded0476f7ece |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 0100879X |
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