دورية أكاديمية
IL-4Rα-Dependent Alternative Activation of Macrophages Is Not Decisive for Mycobacterium tuberculosis Pathology and Bacterial Burden in Mice
| العنوان: | IL-4Rα-Dependent Alternative Activation of Macrophages Is Not Decisive for Mycobacterium tuberculosis Pathology and Bacterial Burden in Mice |
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| المؤلفون: | Guler, Reto, Parihar, Suraj P, Savvi, Suzana, Logan, Erin, Schwegmann, Anita, Roy, Sugata, Nieuwenhuizen, Natalie E, Ozturk, Mumin, Schmeier, Sebastian, Suzuki, Harukazu, Brombacher, Frank |
| المصدر: | PLoS One ; http://journals.plos.org/plosoneTest |
| بيانات النشر: | Public Library of Science University of Cape Town Faculty of Health Sciences Division of Immunology |
| سنة النشر: | 2015 |
| المجموعة: | University of Cape Town: OpenUCT |
| مصطلحات موضوعية: | Macrophages, Mycobacterium tuberculosis, T cells, Histopathology, Alveolar macrophages, Spleen, Tuberculosis, Mycobacterium bovis |
| الوصف: | Classical activation of macrophages (caMph or M1) is crucial for host protection against Mycobacterium tuberculosis ( Mtb ) infection. Evidence suggests that IL-4/IL-13 alternatively activated macrophages (aaMph or M2) are exploited by Mtb to divert microbicidal functions of caMph. To define the functions of M2 macrophages during tuberculosis (TB), we infected mice deficient for IL-4 receptor α on macrophages (LysM cre IL-4Rα -/lox ) with Mtb . We show that absence of IL-4Rα on macrophages does not play a major role during infection with Mtb H37Rv, or the clinical Beijing strain HN878. This was demonstrated by similar mortality, bacterial burden, histopathology and T cell proliferation between infected wild-type (WT) and LysM cre IL-4Rα -/lox mice. Interestingly, we observed no differences in the lung expression of inducible nitric oxide synthase (iNOS) and Arginase 1 (Arg1), well-established markers for M1/M2 macrophages among the Mtb -infected groups. Kinetic expression studies of IL-4/IL-13 activated bone marrow-derived macrophages (BMDM) infected with HN878, followed by gene set enrichment analysis, revealed that the MyD88 and IL-6, IL-10, G-CSF pathways are significantly enriched, but not the IL-4Rα driven pathway. Together, these results suggest that IL-4Rα-macrophages do not play a central role in TB disease progression. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | http://hdl.handle.net/11427/16290Test; http://dx.doi.org/10.1371/journal.pone.0121070Test; https://open.uct.ac.za/bitstream/11427/16290/1/Guler_IL_4Ra_Dependent_Alternative_2015.pdfTest |
| DOI: | 10.1371/journal.pone.0121070 |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0121070Test http://hdl.handle.net/11427/16290Test https://open.uct.ac.za/bitstream/11427/16290/1/Guler_IL_4Ra_Dependent_Alternative_2015.pdfTest |
| حقوق: | This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ; http://creativecommons.org/licenses/by/4.0Test ; © 2015 Guler et al |
| رقم الانضمام: | edsbas.BC8C2BA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0121070 |
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