رسالة جامعية

The Role Of Hydroxamic Acids In Take-all Resistance

التفاصيل البيبلوغرافية
العنوان: The Role Of Hydroxamic Acids In Take-all Resistance
المؤلفون: Wilkes, Meredith Ann
بيانات النشر: Department of Agricultural Chemistry & Soil Science
Faculty of Agriculture
The University of Sydney
سنة النشر: 1997
المجموعة: The University of Sydney: Sydney eScholarship Repository
مصطلحات موضوعية: Grain -- Diseases and pests, Hydroxamic acids, Take-all disease, Wheat -- Disease and pest resistance
الوصف: The occurrence of hydroxamic acids (Hx) and their affects on take-all have been investigated in this study. An improved HPLC procedure for the separation and quantification of Hx in wheat, rye and triticale roots was established. This method completely separated 2,4-Dihydroxy—1,4— benzoxazin—3-one (DIBOA), 2 , 4-Dihydroxy - 7- methoxy - 1 4- benzoxazin -3-one (DIMBOA), 2(3)-benzoxazolinone (BOA) and 6- methoxybenzoxazolinone (MBOA) within 17 min. DIMBOA was the only Hx found in wheat roots, whereas both DIMBOA and DIBOA were present in the roots of triticale and rye. The Hx content of whole roots of wheat, rye and triticale reached a maximum 3 to 4 days after germination, depending on species. The DIMBOA content of wheat roots ranged from 0.4 to 1.5 umoles / g f.wt in the varieties studied. The DIMBOA content of the triticale varieties ranged from 0.9 to 2.0 umoles/ g f.wt, and DIBOA from 0.26 to 1.1 umoles / g f.wt. DIMBOA concentrations in rye roots ranged from 0.3 to 0.5 umoles/ g f.wt, whereas DIBOA levels ranged from zero to 1.1 umoles/g.f.wt. The Hx content of Wheat, rye and triticale roots was highest in the youngest parts of the root. The root tip of these cereals always contained significantly higher levels of Hx than the older parts of the root. When extracts prepared from triticale and rye roots were incorporated into the nutrient media, growth of two isolates of Gaeumannomyces graminis var.tritici (th) (E31 and WP 28) was inhibited. Similar extracts prepared from wheat did not inhibit the growth of th. The fungal strain WP 28 actually grew more rapidly on medium containing extracts from wheat (cv. Sunstar) roots. The inhibitory effect of triticale and rye extracts was attributed to the presence of DIBOA. The inhibitory effect of these particular extracts correlated to the resistance of the respective plant to take-all in the field as reported by Hollins et al . (1986). Hydroxamic acids inhibited the growth of th when incorporated into the growth media. DIBOA and BOA significantly inhibited the growth ...
نوع الوثيقة: doctoral or postdoctoral thesis
وصف الملف: application/pdf
اللغة: English
العلاقة: https://hdl.handle.net/2123/27618Test
الإتاحة: https://hdl.handle.net/2123/27618Test
حقوق: The author retains copyright of this thesis. It may only be used for the purposes of research and study. It must not be used for any other purposes and may not be transmitted or shared with others without prior permission.
رقم الانضمام: edsbas.131FE6A5
قاعدة البيانات: BASE