رسالة جامعية
Desarrollo de un sistema de liberación basado en nanopartículas de quitosano para la optimización del uso terapeútico de interferón alfa
| العنوان: | Desarrollo de un sistema de liberación basado en nanopartículas de quitosano para la optimización del uso terapeútico de interferón alfa |
|---|---|
| المؤلفون: | Cánepa, Camila Belén |
| المساهمون: | Sosnik, Alejandro, Imperiale, Julieta C., Biglione, Mirna, Desimone, Martín, Castro, Guillermo, Martinez Peralta, Liliana |
| بيانات النشر: | Facultad de Farmacia y Bioquímica Universidad de Buenos Aires-Facultad de Farmacia y Bioquímica |
| سنة النشر: | 2018 |
| المجموعة: | Repositorio Digital Institucional - Universidad de Buenos Aires (RDI UBA) |
| مصطلحات موضوعية: | Nanopartículas, Vía Oral, Antiviral, Antitumoral, Interferón alfa, Ciencias de la vida |
| الوصف: | Interferon alpha (IFN?) is protein approved for the treatment of viral and oncological\npathologies, which must be administered parenterally. Our aim was to develop a delivery\nsystem for IFN?-2b by means of a simple, reproducible and economical methodology that\nwould enable its oral administration. Spherical chitosan nanoparticles (CTNPs) of 47 nm were\nobtained by ionotropic gelation. These were stable at pH 5.0-7.0 and mucoadhesive. In\naddition, CTNPs were safe and showed a moderate permeability through colonic cells in vitro.\nThe encapsulation efficiency of the drug loaded system (CT-IFNNPs) was 98%; the\nphysicochemical characteristics were not altered with respect to the unloaded CTNPs. In vitro\nantiviral activity of the CT-IFNNPs was analogous to that of the commercial IFN?. After oral\nadministration of CT-IFNNPs (0,3MIU) to Balb/C mice, IFN? was detected in plasma.\nFurthermore, 3 daily doses of CT-IFNNPs induced the production of pro-inflammatory\ncytokines in spleen, to a greater extent than the pristine IFN? administered subcutaneously,\nsuggesting biological functionality of the drug. Thus, we report for the first time the\ndevelopment of a formulation that enabled the absorption of orally administered IFN?,\nelucidating a new avenue for non-invasive and eventually safer administration routes for\nhighly labile biological drugs, compared to conventional parenteral therapy. ; Fil: Cánepa, Camila Belén. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Buenos Aires, Argentina ; El interferón alfa (IFN?), es una proteína aprobada para tratar patologías virales y oncológicas\nque debe administrarse por vía parenteral. Nos propusimos desarrollar un sistema portador\nde IFN?-2b mediante una metodología simple, reproducible y económica que posibilite su\nadministración oral. Se obtuvieron por gelificación ionotrópica nanopartículas de quitosano\n(NPsCT) de 47nm, esféricas, estables a pH 5,0-7,0 y mucoadhesivas. Además, resultaron\nseguras y de permeabilidad moderada a través de células ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| وصف الملف: | application/pdf |
| اللغة: | Spanish; Castilian |
| العلاقة: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_2932Test; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_2932.dir/2932.PDFTest |
| الإتاحة: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_2932Test http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_2932.dir/2932.PDFTest |
| حقوق: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by-nc-nd/2.5/arTest/ |
| رقم الانضمام: | edsbas.469872A3 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |