Cell cycle‐dependent recruitment of HDAC‐1 correlates with deacetylation of histone H4 on an Rb–E2F target promoter
| العنوان: | Cell cycle‐dependent recruitment of HDAC‐1 correlates with deacetylation of histone H4 on an Rb–E2F target promoter |
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| المؤلفون: | Irina Naguibneva, Linda L. Pritchard, Marion Mathieu, Annick Harel-Bellan, Slimane Ait-Si-Ali, Philippe Robin, Roger Ferreira |
| المصدر: | EMBO reports. 2:794-799 |
| بيانات النشر: | EMBO, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Time Factors, Blotting, Western, Cell Cycle Proteins, Histone Deacetylase 1, Biology, Retinoblastoma Protein, Biochemistry, Histone Deacetylases, Histones, Histone H4, Mice, Histone H1, Histone H2A, Genetics, Animals, Histone code, Promoter Regions, Genetic, Molecular Biology, Cells, Cultured, Histone deacetylase 5, HDAC11, Lysine, Scientific Reports, Cell Cycle, Acetylation, 3T3 Cells, Blotting, Northern, Precipitin Tests, Molecular biology, Chromatin, E2F Transcription Factors, DNA-Binding Proteins, Histone methyltransferase, Histone deacetylase, Cell Division, Protein Binding, Transcription Factors |
| الوصف: | The transcription factor E2F, which is a key element in the control of cell proliferation, is repressed by Rb and other pocket proteins in growth-arrested differentiating cells, as well as in proliferating cells when they progress through early G1. It is not known whether similar mechanisms are operative in the two situations. A body of data suggests that E2F repression by pocket proteins involves class I histone deacetylases (HDACs). It has been hypothesized that these enzymes are recruited to E2F target promoters where they deacetylate histones. Here we have tested this hypothesis directly by using formaldehyde cross-linked chromatin immunoprecipitation (XChIP) assays to evaluate HDAC association in living cells. Our data show that a histone deacetylase, HDAC-1, is stably bound to an E2F target promoter during early G1 in proliferating cells and released at the G1-S transition. In addition, our results reveal an inverse correlation between HDAC-1 recruitment and histone H4 acetylation on specific lysines. |
| تدمد: | 1469-3178 1469-221X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68af1d0bbb3fd4555dc69af209211287Test https://doi.org/10.1093/embo-reports/kve173Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....68af1d0bbb3fd4555dc69af209211287 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14693178 1469221X |
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