RNA‐directed DNA methylation requires an AGO4‐interacting member of the SPT5 elongation factor family
| العنوان: | RNA‐directed DNA methylation requires an AGO4‐interacting member of the SPT5 elongation factor family |
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| المؤلفون: | Natacha Bies-Etheve, Richard Cooke, Thierry Lagrange, Claire Picart, Dominique Pontier, Danielle Vega, Sylvie Lahmy |
| المصدر: | EMBO reports. 10:649-654 |
| بيانات النشر: | EMBO, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Chromosomal Proteins, Non-Histone, Molecular Sequence Data, Scientific Report, Arabidopsis, Biology, Models, Biological, Biochemistry, chemistry.chemical_compound, RNA polymerase, Genetics, Amino Acid Sequence, RNA, Small Interfering, Molecular Biology, RNA-Directed DNA Methylation, RNA polymerase V, Base Sequence, Arabidopsis Proteins, RNA, DNA Methylation, Argonaute, Elongation factor, RNA silencing, chemistry, RNA, Plant, Argonaute Proteins, Mutation, DNA methylation, Transcriptional Elongation Factors, Protein Binding |
| الوصف: | Recent studies have identified a conserved WG/GW-containing motif, known as the Argonaute (AGO) hook, which is involved in the recruitment of AGOs to distinct components of the eukaryotic RNA silencing pathways. By using this motif as a model to detect new components in plant RNA silencing pathways, we identified SPT5-like, a plant-specific AGO4-interacting member of the nuclear SPT5 (Suppressor of Ty insertion 5) RNA polymerase (RNAP) elongation factor family that is characterized by the presence of a carboxy-terminal extension with more than 40 WG/GW motifs. Knockout SPT5-like mutants show a decrease in the accumulation of several 24-nt RNAs and hypomethylation at different loci revealing an implication in RNA-directed DNA methylation (RdDM). Here, we propose that SPT5-like emerged in plants as a facultative RNAP elongation factor. Its plant-specific origin and role in RdDM might reflect functional interactions with plant-specific RNA Pols required for RdDM. |
| تدمد: | 1469-3178 1469-221X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::babde652e366fb553e347f91cb8d0136Test https://doi.org/10.1038/embor.2009.31Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....babde652e366fb553e347f91cb8d0136 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14693178 1469221X |
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