Sensor NLR immune proteins activate oligomerization of their NRC helpers in response to plant pathogens

التفاصيل البيبلوغرافية
العنوان: Sensor NLR immune proteins activate oligomerization of their NRC helpers in response to plant pathogens
المؤلفون: Contreras, Mauricio P, Pai, Hsuan, Tumtas, Yasin, Duggan, Cian, Yuen, Enoch Lok Him, Cruces, Angel Vergara, Kourelis, Jiorgos, Ahn, Hee-Kyung, Lee, Kim-Teng, Wu, Chih-Hang, Bozkurt, Tolga O, Derevnina, Lida, Kamoun, Sophien
المساهمون: Contreras, Mauricio P [0000-0001-6002-0730], Pai, Hsuan [0000-0002-7284-6131], Tumtas, Yasin [0000-0001-7656-6340], Duggan, Cian [0000-0001-7302-7472], Yuen, Enoch Lok Him [0000-0002-7933-0605], Cruces, Angel Vergara [0000-0003-0222-203X], Kourelis, Jiorgos [0000-0002-9007-1333], Ahn, Hee-Kyung [0000-0002-8884-0156], Lee, Kim-Teng [0000-0001-6498-9733], Wu, Chih-Hang [0000-0003-1616-1872], Bozkurt, Tolga O [0000-0003-0507-6875], Derevnina, Lida [0000-0001-7983-4187], Kamoun, Sophien [0000-0002-0290-0315], Apollo - University of Cambridge Repository
بيانات النشر: EMBO, 2023.
سنة النشر: 2023
مصطلحات موضوعية: Mammals, resistosome, immune receptor, Inflammasomes, NLR Proteins, Plants, Immunity, Innate, NB-LRR, inflammasome, Animals, Plant Immunity, NOD-like receptor, Plant Proteins, Plant Diseases
الوصف: Funder: EC¦European Research Council (ERC); Id: http://dx.doi.org/10.13039/501100000781Test
Funder: Gatsby Charitable Foundation (GATSBY); Id: http://dx.doi.org/10.13039/501100000324Test
Funder: John Innes Foundation (JIF); Id: http://dx.doi.org/10.13039/501100004034Test
Nucleotide-binding domain leucine-rich repeat (NLR) immune receptors are important components of plant and metazoan innate immunity that can function as individual units or as pairs or networks. Upon activation, NLRs form multiprotein complexes termed resistosomes or inflammasomes. Although metazoan paired NLRs, such as NAIP/NLRC4, form hetero-complexes upon activation, the molecular mechanisms underpinning activation of plant paired NLRs, especially whether they associate in resistosome hetero-complexes, is unknown. In asterid plant species, the NLR required for cell death (NRC) immune receptor network is composed of multiple resistance protein sensors and downstream helpers that confer immunity against diverse plant pathogens. Here, we show that pathogen effector-activation of the NLR proteins Rx (confers virus resistance), and Bs2 (confers bacterial resistance) leads to oligomerization of their helper NLR, NRC2. Activated Rx does not oligomerize or enter into a stable complex with the NRC2 oligomer and remains cytoplasmic. In contrast, activated NRC2 oligomers accumulate in membrane-associated puncta. We propose an activation-and-release model for NLRs in the NRC immune receptor network. This points to a distinct activation model compared with mammalian paired NLRs.
وصف الملف: application/pdf; text/xml
DOI: 10.17863/cam.92322
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9aa728931ec91c21eebaec60dee5efcTest
رقم الانضمام: edsair.doi.dedup.....a9aa728931ec91c21eebaec60dee5efc
قاعدة البيانات: OpenAIRE