Chondroitin sulphate‐modified neuropilin 1 is expressed in human tumour cells and modulates 3D invasion in the U87MG human glioblastoma cell line through a p130Cas‐mediated pathway
| العنوان: | Chondroitin sulphate‐modified neuropilin 1 is expressed in human tumour cells and modulates 3D invasion in the U87MG human glioblastoma cell line through a p130Cas‐mediated pathway |
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| المؤلفون: | Ian Zachary, Pauliina Lehtolainen, Paul Frankel, Giovanna M. D'Abaco, Lili Cheng, Caroline Pellet-Many, Michelle Tickner |
| المصدر: | EMBO reports. 9:983-989 |
| بيانات النشر: | EMBO, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Swine, Molecular Sequence Data, Scientific Report, Biology, Biochemistry, Mice, chemistry.chemical_compound, Cell Line, Tumor, Neuropilin 1, Genetics, Animals, Humans, Gene silencing, Neoplasm Invasiveness, Amino Acid Sequence, Molecular Biology, Chondroitin Sulfates, Mesenchymal stem cell, Tyrosine phosphorylation, Molecular biology, Neuropilin-1, Hedgehog signaling pathway, Rats, Cell biology, Vascular endothelial growth factor, Crk-Associated Substrate Protein, chemistry, Cell culture, RNA Interference, Signal transduction, Glioblastoma, Signal Transduction |
| الوصف: | Neuropilin 1 (NRP1), a non-tyrosine kinase receptor for vascular endothelial growth factor and class 3 Semaphorins, is highly expressed in many human tumour cell lines, but its function is poorly understood. Here, we describe the expression of a new chondroitin sulphate-modified NRP1 (NRP1-CS) in human tumour cell lines. Expression of a non-modifiable NRP1 mutant (S612A) in U87MG human glioma cells results in enhanced invasion in three dimensions (3D), whereas wild-type NRP1 has no effect. Furthermore, the S612A NRP1 cells show a significant increase in p130Cas tyrosine phosphorylation compared with control and wild-type NRP1 cells. Silencing of p130Cas in S612A NRP1 cells resulted in a loss of increased invasive phenotype. Interestingly, p130Cas silencing does not inhibit basal 3D invasion, but leads to a mesenchymal to amoeboid transition. Biopsies from both low- and high-grade human gliomas show strong expression of NRP1, and little expression of NRP1-CS. Our data establish distinct roles for NRP1 and NRP1-CS in modulating a new NRP1-p130Cas signalling pathway contributing to glioblastoma cell invasion in 3D. |
| تدمد: | 1469-3178 1469-221X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f44f88930198d0d5548e67b4b129a867Test https://doi.org/10.1038/embor.2008.151Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f44f88930198d0d5548e67b4b129a867 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14693178 1469221X |
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