دورية أكاديمية
A sensitive paper-based lateral flow immunoassay platform using engineered cellulose-binding protein linker fused with antibody-binding domains
| العنوان: | A sensitive paper-based lateral flow immunoassay platform using engineered cellulose-binding protein linker fused with antibody-binding domains |
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| المؤلفون: | Yang, J.M., Kim, K.R., Jeon, S., Cha, H.J., Kim, C.S. |
| المساهمون: | Jeon, S., Cha, H.J. |
| بيانات النشر: | ELSEVIER SCIENCE SA |
| سنة النشر: | 2021 |
| المجموعة: | Pohang University of Science and Technology (POSTECH): Open Access System for Information Sharing (OASIS) |
| مصطلحات موضوعية: | ORIENTED IMMOBILIZATION, FUSION PROTEIN, AFFINITY, SURFACES, DESIGN, VIRUS, Lateral flow immunoassay, Cellulose membrane, Cellulose-binding module, Antibody-binding domain, Sensitivity |
| الوصف: | An advanced paper-based LFIA using engineered cellulose-binding protein linker fused with antibody-binding domains was developed to resolve problems associated with conventional LFIA strip, including the limitation of quantitative analysis and low sensitivity. CBP31-BC linker was prepared by genetically fusing the cellulose binding modules of family 3 (CBM3) and family 1 (CBM1) with antibody-binding B and C domains of Protein A. Cellulose-binding analysis showed that the addition of two CBMs led to high cellulose-binding capacity in CBP31-BC. Transmission electron microscopy and quartz crystal microbalance analyses demonstrated that the cellulose-binding ability of CBP31-BC enabled the BC domain to be efficiently exposed, allowing about 6-fold higher antibody-binding efficiency (similar to 32.5 %) than that (similar to 5.5 %) of sole BC. To evaluate the feasibility of the CBP31-BC linker-employed LFIA platform, an immunoassay of prostate-specific antigen (PSA) was performed as a model reaction. The CBP31-BC-employed paper-based LFIA detected PSA at levels as low as 0.25 ng/mL in 20 min, which is about 10-fold more sensitive to conventional LFIAs that use simple physical adsorption. CBP31-BC-employed LFIA also exhibited a lower detection range of 0.25-2.5 ng/mL when compared to conventional LFIAs. These results might be due to efficient oriented immobilization of the capture antibody by CBP31-BC linker on cellulose membrane. Thus, our developed CBP31-BC linker-employed paper-based LFIA platform holds great promise for the detection of target markers due to significantly enhanced sensitivity. ; 1 ; 1 ; N ; scie ; scopus |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0925-4005 |
| العلاقة: | SENSORS AND ACTUATORS B-CHEMICAL; Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation; Chemistry; https://oasis.postech.ac.kr/handle/2014.oak/105166Test; 43552; SENSORS AND ACTUATORS B-CHEMICAL, v.329; 000612042400008; 2-s2.0-85095757025 |
| DOI: | 10.1016/j.snb.2020.129099 |
| الإتاحة: | https://doi.org/10.1016/j.snb.2020.129099Test https://oasis.postech.ac.kr/handle/2014.oak/105166Test |
| رقم الانضمام: | edsbas.A2AAB6BD |
| قاعدة البيانات: | BASE |
| تدمد: | 09254005 |
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| DOI: | 10.1016/j.snb.2020.129099 |